NYMU Taipei/Lab Notes/2007 8 9
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
| Line 7: | Line 7: | ||
*Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells) | *Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells) | ||
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2 | **Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2 | ||
| - | **Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again) | + | **Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 <font color="red">(Transformation again)</font> |
Latest revision as of 13:13, 9 August 2007
- Transformation results
- [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : Only 1 colony
- [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : 8 colonies
- Pick single colony and incubate at LB plate
- Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
- Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
- Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)
