From 2007.igem.org

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	'''Team 1 - Repressilator'''		'''Team 1 - Repressilator'''
-	Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations. We will also test the effect of extracellularly adding autoinducer on synchronization.<br>	+	Our project consists of a two-gene oscillator with coupling by an autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations under different cell densities.<br>
	[[McGill/Background|Background]]		[[McGill/Background|Background]]

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Revision as of 00:36, 16 May 2007

Contents 1 Message Board (temporary) 2 In the Lab 3 The Team 4 Project Overviews

Message Board (temporary)

I'll chop up Adam's PDF on my break today to put it into the Protocols section. Tim

Hello Tim,

we need a lab notebook page, protocols page and team members page, etc.

PS: Everyone, get a lab notebook! We're making our cells chemically competent tomorrow so if you want to see/help out then show up around 10:30. Horiavulpe 16:41, 14 May 2007 (EDT)

Note from iGEM: Please use the format "McGill/Page Name" for all the pages your team uses. I fixed teh Lab Protocols, the Lab Notebook, and the Team Roster links. Thanks, - Randy

In the Lab

Lab Protocols
Lab Notebook

The Team

Mailing List
Team Roster

Project Overviews

Team 1 - Repressilator Our project consists of a two-gene oscillator with coupling by an autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations under different cell densities.

Background

Team 2 - Fluorescence Complimentation This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that revesibly associate.