Melb:Plan:GFP Fluorescent reporter

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LacZ reporter

  1. Ligate LacZ coding region to gentamycin RBS part. (Done?)
  2. Transform and select for appropriate construct using Kan/Gen plate. (Done?)
  3. Liquid culture and screen via restriction digest (EcoRI and SpeI), digest double terminator (P1 1G) simultaneously (EcoRI and XbaI) (Monday 13/08/07)
  4. Make glycerol stock of RBS-LacZ containing cells. (Monday 13/08/07)
  1. Ligate the RBS-LacZ construct into double terminator plasmid. (Monday 13/08/07)
  2. Transform ligation and select for construct
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