ETHZ/labnotes

From 2007.igem.org

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* Transformations of J23100, J37033, Q04400, Q04510
* Transformations of J23100, J37033, Q04400, Q04510
* Control Restrictions (step after "MINIPREP") <br>R0040, R0051, Q04121, C0053
* Control Restrictions (step after "MINIPREP") <br>R0040, R0051, Q04121, C0053
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* Overnight culture (Top 10 cells)
* Overnight culture (Top 10 cells)
* Control Restrictions (didn't work)
* Control Restrictions (didn't work)
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| Tue, 14. Aug. 2007           
| Tue, 14. Aug. 2007           
| Morning Shift: <br>
| Morning Shift: <br>
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* Prepare competent cells for J23100, J37033, Q04400, Q04510                       
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* Start Preparing competent cells for J23100, J37033, Q04400, Q04510                       
-
* Transformations of J23100, J37033, Q04400, Q04510
+
* Control Restrictions (step after "MINIPREP") <br>R0040, R0051, Q04121, C0053
* Control Restrictions (step after "MINIPREP") <br>R0040, R0051, Q04121, C0053
-
|                      
+
| Evening Shift: <br>
 +
* Transformations of J23100, J37033, Q04400, Q04510
 +
* Gel Electrophoresis of R0040, R0051, Q04121, C0053
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|                       
| * Tim (from 17:30 until it's done)<br> Martin<br> Christos (11:00-13:00, 18:00-00:00)
| * Tim (from 17:30 until it's done)<br> Martin<br> Christos (11:00-13:00, 18:00-00:00)
|-
|-

Revision as of 19:17, 13 August 2007

Part/Plasmid Numberings

The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.

  1. [http://partsregistry.org/Part:BBa_B0034 B0034]
  2. [http://partsregistry.org/Part:BBa_R0062 R0062]
  3. [http://partsregistry.org/Part:BBa_R0053 R0053]
  4. [http://partsregistry.org/Part:BBa_J23100 J23100]
  5. [http://partsregistry.org/Part:BBa_J37033 J37033]
  6. [http://partsregistry.org/Part:BBa_E0434 E0434]
  7. [http://partsregistry.org/Part:BBa_B0015 B0015]
  8. [http://partsregistry.org/Part:BBa_Q04400 Q04400]
  9. [http://partsregistry.org/Part:BBa_R0010 R0010]
  10. [http://partsregistry.org/Part:BBa_E0422 E0422]
  11. [http://partsregistry.org/Part:BBa_R0040 R0040]
  12. [http://partsregistry.org/Part:BBa_R0051 R0051]
  13. [http://partsregistry.org/Part:BBa_Q04121 Q04121]
  14. [http://partsregistry.org/Part:BBa_C0053 C0053]
  15. [http://partsregistry.org/Part:BBa_Q04510 Q4510]

Week 1

Date TODO's Completed People
Mon, 06. Aug. 2007
Tue, 07. Aug. 2007
Wed, 08. Aug. 2007
Thu, 09. Aug. 2007
Fri, 10. Aug. 2007
  • 7 working parts/plasmids (step after "DIGESTS"):
    (B0034, R0062, R0053, E0434, B0015, R0010, E0422)
  • 4 parts/plasmids minipreped:
    (R0040, R0051, Q04121, C0053)

Christos
Markus
Stefan

Sat, 11. Aug. 2007
Sun, 12. Aug. 2007
labwork cancelled

Christos
Joe
Martin

Week 2

Date TODO's Completed People
Mon, 13. Aug. 2007
start at 3 pm
  • Prepare competent cells
  • Transformations of J23100, J37033, Q04400, Q04510
  • Control Restrictions (step after "MINIPREP")
    R0040, R0051, Q04121, C0053
  • Overnight culture (Top 10 cells)
  • Control Restrictions (didn't work)
Martin
Markus
Christos
Tim
Tue, 14. Aug. 2007 Morning Shift:
  • Start Preparing competent cells for J23100, J37033, Q04400, Q04510
  • Control Restrictions (step after "MINIPREP")
    R0040, R0051, Q04121, C0053
Evening Shift:
  • Transformations of J23100, J37033, Q04400, Q04510
  • Gel Electrophoresis of R0040, R0051, Q04121, C0053
* Tim (from 17:30 until it's done)
Martin
Christos (11:00-13:00, 18:00-00:00)
Wed, 15. Aug. 2007
  • Miniprep of competent cells (J23100, J37033, Q04400, Q04510)
  • Ligation (step: "LINK ASSEMBLY"):
    R0053 + E0422
    R0010 + E0422
    R0010 + E0434
    S/P: R0053, R0010
    X/P: E0422, E0434
* Tim (from 17:30 until it's done)
Martin
Christos (11:00-13:00, 18:00-00:00)
Thu, 16. Aug. 2007

Christos (11:00-13:00, 18:00-00:00)
Martin

Fri, 17. Aug. 2007 Martin
Nan (from 18:00 till 00:00)
Sat, 18. Aug. 2007 Martin
Nan
Sun, 19. Aug. 2007 Martin
Nan
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