Glasgow/Wetlab/Biobricks
From 2007.igem.org
(Difference between revisions)
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== BioBrick Reference == | == BioBrick Reference == | ||
{| border="1" cellspacing="0" cellpadding="5" align="center" | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
+ | !Number | ||
!Label | !Label | ||
!Plate | !Plate | ||
Line 9: | Line 10: | ||
!Plasmid | !Plasmid | ||
!Resistance | !Resistance | ||
- | ! | + | !V |
!Description | !Description | ||
+ | !Results | ||
|- | |- | ||
+ | |1 | ||
|4/7A | |4/7A | ||
|4 | |4 | ||
Line 20: | Line 23: | ||
| | | | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |2 | ||
|4/11E | |4/11E | ||
|4 | |4 | ||
Line 29: | Line 34: | ||
| | | | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | | | ||
|4/11C | |4/11C | ||
|4 | |4 | ||
Line 38: | Line 45: | ||
| | | | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |3 | ||
|2/23N | |2/23N | ||
|2 | |2 | ||
Line 47: | Line 56: | ||
|V1005 | |V1005 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |4 | ||
|2/21B | |2/21B | ||
|2 | |2 | ||
Line 55: | Line 66: | ||
|Carbenicillin | |Carbenicillin | ||
|V1005 | |V1005 | ||
- | |Death gene plasmid | + | |Death gene plasmid| |
|- | |- | ||
+ | |5 | ||
|2/23P | |2/23P | ||
|2 | |2 | ||
Line 65: | Line 77: | ||
|V1005 | |V1005 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |6 | ||
|2/2B | |2/2B | ||
|2 | |2 | ||
Line 74: | Line 88: | ||
|V1005 | |V1005 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |7 | ||
|2/6J | |2/6J | ||
|2 | |2 | ||
Line 83: | Line 99: | ||
|V1005 | |V1005 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |8 | ||
|2/12J | |2/12J | ||
|2 | |2 | ||
Line 92: | Line 110: | ||
|V1005 | |V1005 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |9 | ||
|3/20G | |3/20G | ||
|3 | |3 | ||
Line 101: | Line 121: | ||
|V1015 | |V1015 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |10 | ||
|3/20I | |3/20I | ||
|3 | |3 | ||
Line 110: | Line 132: | ||
|V1015 | |V1015 | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |11 | ||
|5/6B | |5/6B | ||
|5 | |5 | ||
Line 119: | Line 143: | ||
| | | | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |12 | ||
|5/6C | |5/6C | ||
|5 | |5 | ||
Line 128: | Line 154: | ||
| | | | ||
|Death gene plasmid | |Death gene plasmid | ||
+ | | | ||
|- | |- | ||
+ | |13 | ||
|4/5I | |4/5I | ||
|4 | |4 | ||
Line 137: | Line 165: | ||
| | | | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |14 | ||
|4/5O | |4/5O | ||
|4 | |4 | ||
Line 146: | Line 176: | ||
| | | | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |15 | ||
|4/6B | |4/6B | ||
|4 | |4 | ||
Line 155: | Line 187: | ||
| | | | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |16 | ||
|4/6D | |4/6D | ||
|4 | |4 | ||
Line 163: | Line 197: | ||
|Kanamycin | |Kanamycin | ||
| | | | ||
- | |High copy death gene | + | |High copy death gene| |
|- | |- | ||
+ | |17 | ||
|4/6F | |4/6F | ||
|4 | |4 | ||
Line 173: | Line 208: | ||
| | | | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |18 | ||
|4/5K | |4/5K | ||
|4 | |4 | ||
Line 182: | Line 219: | ||
| | | | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |19 | ||
|4/5M | |4/5M | ||
|4 | |4 | ||
Line 191: | Line 230: | ||
| | | | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |20 | ||
|4/4J | |4/4J | ||
|4 | |4 | ||
Line 199: | Line 240: | ||
|Chloramphenicol | |Chloramphenicol | ||
|V1009 | |V1009 | ||
- | |High copy death gene | + | |High copy death gene| |
|- | |- | ||
+ | |21 | ||
|4/4L | |4/4L | ||
|4 | |4 | ||
Line 209: | Line 251: | ||
|V1009 | |V1009 | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |22 | ||
|4/4N | |4/4N | ||
|4 | |4 | ||
Line 218: | Line 262: | ||
|V1009 | |V1009 | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |23 | ||
|4/4P | |4/4P | ||
|4 | |4 | ||
Line 227: | Line 273: | ||
|V1009 | |V1009 | ||
|High copy death gene | |High copy death gene | ||
+ | | | ||
|- | |- | ||
+ | |24 | ||
|3/19A | |3/19A | ||
|3 | |3 | ||
Line 236: | Line 284: | ||
|V1013 | |V1013 | ||
|Strong constitutive promoter | |Strong constitutive promoter | ||
+ | | | ||
|- | |- | ||
+ | |25 | ||
|1/9G | |1/9G | ||
|1 | |1 | ||
Line 245: | Line 295: | ||
|V1004 | |V1004 | ||
|HSL + LuxR Inducible | |HSL + LuxR Inducible | ||
+ | | | ||
|- | |- | ||
+ | |26 | ||
|1/16P | |1/16P | ||
|1 | |1 | ||
Line 254: | Line 306: | ||
|V1009 | |V1009 | ||
|IPTG: inducible promoter + RBS | |IPTG: inducible promoter + RBS | ||
+ | | | ||
|- | |- | ||
+ | |27 | ||
|1/5H | |1/5H | ||
|1 | |1 | ||
Line 263: | Line 317: | ||
|V100 | |V100 | ||
|GFP mutant no promoter (3b) | |GFP mutant no promoter (3b) | ||
+ | | | ||
|- | |- | ||
+ | |28 | ||
|1/1G | |1/1G | ||
|1 | |1 | ||
Line 272: | Line 328: | ||
| | | | ||
|Double terminator | |Double terminator | ||
+ | | | ||
|- | |- | ||
+ | |29 | ||
|1/1I | |1/1I | ||
|1 | |1 | ||
Line 281: | Line 339: | ||
| | | | ||
|Double terminator | |Double terminator | ||
+ | | | ||
|- | |- | ||
+ | |30 | ||
|3/3O | |3/3O | ||
|3 | |3 | ||
Line 290: | Line 350: | ||
| | | | ||
|Double terminator | |Double terminator | ||
+ | | | ||
|- | |- | ||
+ | |31 | ||
|4/12N | |4/12N | ||
|4 | |4 | ||
Line 299: | Line 361: | ||
| | | | ||
|RBSes | |RBSes | ||
+ | | | ||
|- | |- | ||
+ | |32 | ||
|4/12J | |4/12J | ||
|4 | |4 | ||
Line 308: | Line 372: | ||
| | | | ||
|RBSes | |RBSes | ||
+ | | | ||
|- | |- | ||
+ | |33 | ||
|4/12L | |4/12L | ||
|4 | |4 | ||
Line 317: | Line 383: | ||
| | | | ||
|RBSes | |RBSes | ||
+ | | | ||
|- | |- | ||
+ | |34 | ||
|4/12P | |4/12P | ||
|4 | |4 | ||
Line 326: | Line 394: | ||
| | | | ||
|RBSes | |RBSes | ||
+ | | | ||
|- | |- | ||
+ | |35 | ||
|1/11A | |1/11A | ||
|1 | |1 | ||
Line 335: | Line 405: | ||
| | | | ||
|EYFP,RBS + terminator | |EYFP,RBS + terminator | ||
+ | | | ||
|- | |- | ||
+ | |36 | ||
|1/11G | |1/11G | ||
|1 | |1 | ||
Line 344: | Line 416: | ||
| | | | ||
|ECFP, RBS, Term, +LVA TAG | |ECFP, RBS, Term, +LVA TAG | ||
+ | | | ||
|- | |- | ||
+ | |37 | ||
|1/11C | |1/11C | ||
|1 | |1 | ||
Line 353: | Line 427: | ||
| | | | ||
|EYFP, RBS, Term, + LVA TAG | |EYFP, RBS, Term, + LVA TAG | ||
+ | | | ||
|} | |} |
Revision as of 17:52, 16 August 2007
BioBrick Reference
Number | Label | Plate | Location | Brick | Plasmid | Resistance | V | Description | Results |
---|---|---|---|---|---|---|---|---|---|
1 | 4/7A | 4 | 7A | BBa_p1010 | pSB1A10 | Carbenicillin | Death gene plasmid | ||
2 | 4/11E | 4 | 11E | BBa_p1010 | pSB1A10 | Carbenicillin | Death gene plasmid | ||
4/11C | 4 | 11C | BBa_p1010 | pSB3K3 | Kanamycin | Death gene plasmid | |||
3 | 2/23N | 2 | 23N | BBa_p1010 | pSB1AK3 | Carbenicillin/ Kanamycin | V1005 | Death gene plasmid | |
4 | 2/21B | 2 | 21B | BBa_p1010 | pSB1AC3 | Carbenicillin | V1005 | ||
5 | 2/23P | 2 | 23P | BBa_p1010 | pSB1AT3 | Carbenicillin/Tetracycline | V1005 | Death gene plasmid | |
6 | 2/2B | 2 | 2B | BBa_p1010 | pSB1AC3 | Chloramphenicol | V1005 | Death gene plasmid | |
7 | 2/6J | 2 | 6J | BBa_p1010 | pSB4A3 | Carbenicillin | V1005 | Death gene plasmid | |
8 | 2/12J | 2 | 12J | BBa_p1010 | pSB2K3 | Kanamycin | V1005 | Death gene plasmid | |
9 | 3/20G | 3 | 20G | BBa_p1010 | pSB1A2 | Carbenicillin | V1015 | Death gene plasmid | |
10 | 3/20I | 3 | 20I | BBa_p1010 | pSB1A3 | Carbenicillin | V1015 | Death gene plasmid | |
11 | 5/6B | 5 | 6B | BBa_p1010 | pSB3K3 | Kanamycin | Death gene plasmid | ||
12 | 5/6C | 5 | 6C | BBa_p1010 | pSB1A10 | Carbenicillin | Death gene plasmid | ||
13 | 4/5I | 4 | 5I | BBa_I52001 | pSB4A5 | Carbenicillin | High copy death gene | ||
14 | 4/5O | 4 | 5O | BBa_I52001 | pSB4K5 | Kanamycin | High copy death gene | ||
15 | 4/6B | 4 | 6B | BBa_I52001 | pSB3K5 | Kanamycin | High copy death gene | ||
16 | 4/6D | 4 | 6D | BBa_I52001 | pSB4K5 | Kanamycin | |||
17 | 4/6F | 4 | 6F | BBa_I52001 | pSB4A5 | Carbenicillin | High copy death gene | ||
18 | 4/5K | 4 | 5K | BBa_I52001 | pSB4C5 | Chloramphenicol | High copy death gene | ||
19 | 4/5M | 4 | 5M | BBa_I52001 | pSB4T5 | Tetracycline | High copy death gene | ||
20 | 4/4J | 4 | 4J | BBa_I52001 | pSB3C5 | Chloramphenicol | V1009 | ||
21 | 4/4L | 4 | 4L | BBa_I52001 | pSB4C5 | Chloramphenicol | V1009 | High copy death gene | |
22 | 4/4N | 4 | 4N | BBa_I52001 | pSB3T5 | Tetracylcine | V1009 | High copy death gene | |
23 | 4/4P | 4 | 4P | BBa_I52001 | pSB4T5 | Tetracycline | V1009 | High copy death gene | |
24 | 3/19A | 3 | 19/A | BBa_J23119 | pSB1A2 | Carbenicillin | V1013 | Strong constitutive promoter | |
25 | 1/9G | 1 | 9G | BBa_R0062 | pSB1A2 | Carbenicillin | V1004 | HSL + LuxR Inducible | |
26 | 1/16P | 1 | 16P | BBa_J04500 | pSB1AK3 | Carbenicillin/Kanamycin | V1009 | IPTG: inducible promoter + RBS | |
27 | 1/5H | 1 | 5H | BBa_E0040 | pSB1A2 | Carbenicillin | V100 | GFP mutant no promoter (3b) | |
28 | 1/1G | 1 | 1G | BBa_B0014 | pSB1AK3 | Carbenicillin/Kanamycin | Double terminator | ||
29 | 1/1I | 1 | 1I | BBa_B0015 | pSB1AK3 | Carbenicillin/Kanamycin | Double terminator | ||
30 | 3/3O | 3 | 3O | BBa_B0015 | pSB1AK3 | Carbenicillin/Kanamycin | Double terminator | ||
31 | 4/12N | 4 | 12N | BBa_J6110/J61103 | pSB1A2 | Carbenicillin | RBSes | ||
32 | 4/12J | 4 | 12J | BBa_J6110/J61103 | pSB1A2 | Carbenicillin | RBSes | ||
33 | 4/12L | 4 | 12L | BBa_J6110/J61103 | pSB1A2 | Carbenicillin | RBSes | ||
34 | 4/12P | 4 | 12P | BBa_J6110/J61103 | pSB1A2 | Carbenicillin | RBSes | ||
35 | 1/11A | 1 | 11A | BBa_E0430 | pSB1A2 | Carbenicillin | EYFP,RBS + terminator | ||
36 | 1/11G | 1 | 11G | BBa_E0422 | pSB1A2 | Carbenicillin | ECFP, RBS, Term, +LVA TAG | ||
37 | 1/11C | 1 | 11C | BBa_E0432 | pSB1A2 | Carbenicillin | EYFP, RBS, Term, + LVA TAG |