Melbourne/meeting 10 August 2007
From 2007.igem.org
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== 5:30 Action points from last meeting:== | == 5:30 Action points from last meeting:== | ||
* <b>Alisa& Pat: </b>Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07) | * <b>Alisa& Pat: </b>Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07) | ||
| - | * <b>Jan:</b> Lecture Timetables spread sheet to be emailed to all | + | * <b>Jan:</b> Lecture Timetables spread sheet to be emailed to all->on google groups |
| - | * <b>Pat & Alisa: </b>Plan for red light receptor on wiki | + | * <b>Pat & Alisa: </b>Plan for red light receptor on wiki -(outstanding from 3/8/07) |
| - | * <b>Alisa, Paul & Jan: </b>Review blue light theory | + | * <b>Alisa, Paul & Jan: </b>Review blue light theory - done |
| - | * <b>Jan:</b>Theory for blue light on wiki | + | * <b>Jan:</b>Theory for blue light on wiki-done |
| - | * <b>Jan:</b>ComP fusion to be ordered. | + | * <b>Jan:</b>ComP fusion to be ordered.- 31 August shipping |
* <b>Jan:</b>Production of sequence for blue light receptor? " -designed | * <b>Jan:</b>Production of sequence for blue light receptor? " -designed | ||
| - | * <b>Jan:</b>Plan for blue light on wiki by 17th August | + | * <b>Jan:</b>Plan for blue light on wiki by 17th August- Done (needs more details- coundn't implement this) |
| - | * <b>Jan:<b/>Order red and blue filters. | + | * <b>Jan:<b/>Order red and blue filters.-dropped |
| - | * <b>Paul:</b> Status of #BBa-M30109 & #BBa-V1012 lost in transit? | + | * <b>Paul:</b> Status of #BBa-M30109 & #BBa-V1012 lost in transit?- Phil to chase (one unmarked tube arrived) |
| - | + | ||
* <b>Craig:</b> Alkaline lysis technique for miniprep to be documented | * <b>Craig:</b> Alkaline lysis technique for miniprep to be documented | ||
| - | * <b>Cheng:</b> Daisy to produce competent cells of DB3.1 and Enz- strains. | + | * <b>Cheng:</b> Daisy to produce competent cells of DB3.1 and Enz- strains.- waiting on strains still |
== 5:45 Status Reports: (5 minutes each including any discussion)== | == 5:45 Status Reports: (5 minutes each including any discussion)== | ||
* <b>Alisa: </b> Ligation experiments progress. | * <b>Alisa: </b> Ligation experiments progress. | ||
| - | ** | + | ** Attempting ligation of RBS to LacZ reporter. |
| - | + | ||
| - | + | ||
| - | + | ||
* <b>Pat & Alisa: </b> Red light receptor progress/design/plan- needs? | * <b>Pat & Alisa: </b> Red light receptor progress/design/plan- needs? | ||
** phase 1 osmolarity test of YFP under enz | ** phase 1 osmolarity test of YFP under enz | ||
** phase 2 Red light driving YFP | ** phase 2 Red light driving YFP | ||
| - | ** | + | ** Nothing planned for this week due to academic load. |
* <b>Jan:</b> Blue light receptor design/plan - needs? | * <b>Jan:</b> Blue light receptor design/plan - needs? | ||
| - | ** | + | ** Jan will talk to physics about prism, nothing else planned this week |
| - | + | ||
| - | + | ||
* <b>Phil:</b> Gas vesicles progress/design/plan - needs? | * <b>Phil:</b> Gas vesicles progress/design/plan - needs? | ||
** Plan up to basic biobrick complete, | ** Plan up to basic biobrick complete, | ||
| - | ** First mutagenesis | + | ** First mutagenesis complete: 4 changes sucessfull independently , 4 unsucessfull. Unsuccesssfull changes correlated with Tm of primers will repeat with longer melting periods. Test of primer design systems yeilded varying results, Best(30colonies: 2/3 good) was stratagene program followed by jeremy's(Wehi offset design)(13 colonies 0/3 good) followed by steves (complementary hand design to stratagenes rules)(3 colonies 1/3 good). On this basis Phil had entered statagene designed primers on geneworks order form, Alisa to submit for Friday delivery. |
** Plan for support parts/test harness required. | ** Plan for support parts/test harness required. | ||
** Lac promotor and antibiotic death plasmids required. | ** Lac promotor and antibiotic death plasmids required. | ||
* <b>Kasia:</b> Documentation progress vs last report/ new problems/ needs.-No report | * <b>Kasia:</b> Documentation progress vs last report/ new problems/ needs.-No report | ||
==6.15 New Agenda Items:== | ==6.15 New Agenda Items:== | ||
| - | + | * <b>Alisa:</b> To Introduces Chris Loo. | |
| - | * <b>Alisa:</b> To | + | |
* <b>Phil:</b> Administrative Timetable: (11 Weeks to go) | * <b>Phil:</b> Administrative Timetable: (11 Weeks to go) | ||
| - | * <b>Next meeting: <b/> | + | * <b>Next meeting: <b/> 17th August (Friday) 5.30PM. |
==7:30 End == | ==7:30 End == | ||
Revision as of 21:30, 16 August 2007
Contents |
5:30 Action points from last meeting:
- Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
- Jan: Lecture Timetables spread sheet to be emailed to all->on google groups
- Pat & Alisa: Plan for red light receptor on wiki -(outstanding from 3/8/07)
- Alisa, Paul & Jan: Review blue light theory - done
- Jan:Theory for blue light on wiki-done
- Jan:ComP fusion to be ordered.- 31 August shipping
- Jan:Production of sequence for blue light receptor? " -designed
- Jan:Plan for blue light on wiki by 17th August- Done (needs more details- coundn't implement this)
- Jan:<b/>Order red and blue filters.-dropped
- <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?- Phil to chase (one unmarked tube arrived)
- Craig: Alkaline lysis technique for miniprep to be documented
- Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.- waiting on strains still
5:45 Status Reports: (5 minutes each including any discussion)
- Alisa: Ligation experiments progress.
- Attempting ligation of RBS to LacZ reporter.
- Pat & Alisa: Red light receptor progress/design/plan- needs?
- phase 1 osmolarity test of YFP under enz
- phase 2 Red light driving YFP
- Nothing planned for this week due to academic load.
- Jan: Blue light receptor design/plan - needs?
- Jan will talk to physics about prism, nothing else planned this week
- Phil: Gas vesicles progress/design/plan - needs?
- Plan up to basic biobrick complete,
- First mutagenesis complete: 4 changes sucessfull independently , 4 unsucessfull. Unsuccesssfull changes correlated with Tm of primers will repeat with longer melting periods. Test of primer design systems yeilded varying results, Best(30colonies: 2/3 good) was stratagene program followed by jeremy's(Wehi offset design)(13 colonies 0/3 good) followed by steves (complementary hand design to stratagenes rules)(3 colonies 1/3 good). On this basis Phil had entered statagene designed primers on geneworks order form, Alisa to submit for Friday delivery.
- Plan for support parts/test harness required.
- Lac promotor and antibiotic death plasmids required.
- Kasia: Documentation progress vs last report/ new problems/ needs.-No report
6.15 New Agenda Items:
- Alisa: To Introduces Chris Loo.
- Phil: Administrative Timetable: (11 Weeks to go)
- Next meeting: <b/> 17th August (Friday) 5.30PM.
7:30 End
Igem Administrative Timetable:
| 1 September | Final team rosters due. |
| 1 September | Jamboree attendance fees due $100USD per team
member. |
| 26 October | Project and part documentation due DNA received USA. |
| 26 October | BioBrick Part DNA received by the Registry. |
| 3-4 November | iGEM Competition Jamboree, MIT, USA. |
.
Documentation wiki status:
- Priority should be on plans and experimental report analysis and conclusions-----Alisa .
- If only Pat & Alisa doing lab work not much documentation needed.
- Equipement:
- MissingLas3000 method (in lab notebook)
- Missing DNA measurement protocol (eppendorf)
- Reagents pages incomplete:
- Some location pictures not finished or uploaded.
- Some newer reagents not entered.
- Secondary reagents pages not made:
- LB, Plates, TAE , NZY+ Broth
- Protocol part:
- No connections from Protocols to reagents.
- Ligation protocol missing
- Lab notebook:
- Format uses too many lines to see connections between days.
- Latter part missing links
- Can table of contents be restricted to date level?
- Experimental reports:
- Lacks discussion/conclusion
- Ligation experiment missing
- Lattest few gells not marked up and entered.
- Project overview link broken
- Background:
- GFP missing ,
- Red photo sensor to GFP test harness missing.
- Plan: Only Gas vesicles plan entered.
| Individual | Desired time committment |
|---|---|
| Pat | About 4h/wk Monday afternoon or Tuesday morning |
| Alisa | About 4h/wk - Thursday afternoon |
| Jan | About 10h/pw - flexible weekday mornings |
| Phil | About 18h/pw - Thursday morning, Friday, Saturday or Sunday, |
