Melbourne/meeting 10 August 2007

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< Melbourne(Difference between revisions)
 
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** Plan up to basic biobrick complete,  
** Plan up to basic biobrick complete,  
** First mutagenesis complete: 4 changes sucessfull independently , 4 unsucessfull. Unsuccesssfull changes correlated with Tm of primers will repeat with longer melting periods. Test of primer design systems yeilded varying results, Best(30colonies: 2/3 good) was stratagene program followed by jeremy's(Wehi offset design)(13 colonies 0/3 good)  followed by steves (complementary hand design to stratagenes rules)(3 colonies 1/3 good). On this basis Phil had entered statagene designed primers on geneworks order form, Alisa to submit for Friday delivery.
** First mutagenesis complete: 4 changes sucessfull independently , 4 unsucessfull. Unsuccesssfull changes correlated with Tm of primers will repeat with longer melting periods. Test of primer design systems yeilded varying results, Best(30colonies: 2/3 good) was stratagene program followed by jeremy's(Wehi offset design)(13 colonies 0/3 good)  followed by steves (complementary hand design to stratagenes rules)(3 colonies 1/3 good). On this basis Phil had entered statagene designed primers on geneworks order form, Alisa to submit for Friday delivery.
 +
** Need to check expression of gas vesicles Chris to perform.
** Plan for support parts/test harness required.
** Plan for support parts/test harness required.
** Lac promotor and antibiotic death plasmids required.
** Lac promotor and antibiotic death plasmids required.
* <b>Kasia:</b> Documentation progress vs last report/ new problems/ needs.-No report
* <b>Kasia:</b> Documentation progress vs last report/ new problems/ needs.-No report
==6.15 New Agenda Items:==
==6.15 New Agenda Items:==
-
*      <b>Alisa:</b> To Introduces Chris Loo.
+
*      <b>Alisa:</b> Introduces Chris Loo.
* <b>Phil:</b> Administrative Timetable: (11 Weeks to go)
* <b>Phil:</b> Administrative Timetable: (11 Weeks to go)
* <b>Next meeting: <b/> 17th August (Friday) 5.30PM.
* <b>Next meeting: <b/> 17th August (Friday) 5.30PM.

Latest revision as of 21:48, 16 August 2007

Contents

5:30 Action points from last meeting:

  • Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
  • Jan: Lecture Timetables spread sheet to be emailed to all->on google groups
  • Pat & Alisa: Plan for red light receptor on wiki -(outstanding from 3/8/07)
  • Alisa, Paul & Jan: Review blue light theory - done
  • Jan:Theory for blue light on wiki-done
  • Jan:ComP fusion to be ordered.- 31 August shipping
  • Jan:Production of sequence for blue light receptor? " -designed
  • Jan:Plan for blue light on wiki by 17th August- Done (needs more details- coundn't implement this)
  • Jan:<b/>Order red and blue filters.-dropped
  • <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?- Phil to chase (one unmarked tube arrived)
  • Craig: Alkaline lysis technique for miniprep to be documented
  • Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.- waiting on strains still

5:45 Status Reports: (5 minutes each including any discussion)

  • Alisa: Ligation experiments progress.
    • Attempting ligation of RBS to LacZ reporter.
  • Pat & Alisa: Red light receptor progress/design/plan- needs?
    • phase 1 osmolarity test of YFP under enz
    • phase 2 Red light driving YFP
    • Nothing planned for this week due to academic load.
  • Jan: Blue light receptor design/plan - needs?
    • Jan will talk to physics about prism, nothing else planned this week
  • Phil: Gas vesicles progress/design/plan - needs?
    • Plan up to basic biobrick complete,
    • First mutagenesis complete: 4 changes sucessfull independently , 4 unsucessfull. Unsuccesssfull changes correlated with Tm of primers will repeat with longer melting periods. Test of primer design systems yeilded varying results, Best(30colonies: 2/3 good) was stratagene program followed by jeremy's(Wehi offset design)(13 colonies 0/3 good) followed by steves (complementary hand design to stratagenes rules)(3 colonies 1/3 good). On this basis Phil had entered statagene designed primers on geneworks order form, Alisa to submit for Friday delivery.
    • Need to check expression of gas vesicles Chris to perform.
    • Plan for support parts/test harness required.
    • Lac promotor and antibiotic death plasmids required.
  • Kasia: Documentation progress vs last report/ new problems/ needs.-No report

6.15 New Agenda Items:

  • Alisa: Introduces Chris Loo.
  • Phil: Administrative Timetable: (11 Weeks to go)
  • Next meeting: <b/> 17th August (Friday) 5.30PM.

7:30 End

Igem Administrative Timetable:

1 September Final team rosters due.
1 September Jamboree attendance fees due $100USD per team

member.

26 October Project and part documentation due DNA received USA.
26 October BioBrick Part DNA received by the Registry.
3-4 November iGEM Competition Jamboree, MIT, USA.

.


Documentation wiki status:

    • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
    • If only Pat & Alisa doing lab work not much documentation needed.
    • Equipement:
      • MissingLas3000 method (in lab notebook)
      • Missing DNA measurement protocol (eppendorf)
    • Reagents pages incomplete:
      • Some location pictures not finished or uploaded.
      • Some newer reagents not entered.
    • Secondary reagents pages not made:
      • LB, Plates, TAE , NZY+ Broth
    • Protocol part:
      • No connections from Protocols to reagents.
      • Ligation protocol missing
    • Lab notebook:
      • Format uses too many lines to see connections between days.
      • Latter part missing links
      • Can table of contents be restricted to date level?
    • Experimental reports:
      • Lacks discussion/conclusion
      • Ligation experiment missing
      • Lattest few gells not marked up and entered.
    • Project overview link broken
    • Background:
      • GFP missing ,
      • Red photo sensor to GFP test harness missing.
    • Plan: Only Gas vesicles plan entered.
IndividualDesired time committment
Pat About 4h/wk Monday afternoon or Tuesday morning
Alisa About 4h/wk - Thursday afternoon
Jan About 10h/pw - flexible weekday mornings
Phil About 18h/pw - Thursday morning, Friday, Saturday or Sunday,
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