Melbourne/Parts used

From 2007.igem.org

(Difference between revisions)
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|-
|-
! [[Melbourne/BBa_B0014|P1 1G]]
! [[Melbourne/BBa_B0014|P1 1G]]
-
| BBa_B0014 || Double Terminator || 95 || pSB1AK3 || 3189 || 3284 || Amp, Kan || Confirmed
+
| BBa_B0014 || Double Terminator || 95 || pSB1AK3 || 3189 || 3284 || Amp, Kan || Glycerol stocks in -80
|-
|-
! [[Melbourne/BBa_B0034|P1 3O]]
! [[Melbourne/BBa_B0034|P1 3O]]
-
| BBa_B0034 || RBS || 12 || pSB1A2 || 2079 || 2091 || Amp || Miniprepped/Unconfirmed
+
| BBa_B0034 || RBS || 12 || pSB1A2 || 2079 || 2091 || Amp || Discontinued
|-
|-
! [[Melbourne/BBa_C0051|P1 5G]]
! [[Melbourne/BBa_C0051|P1 5G]]
-
| BBa_C0051 || c1 represser protein || 750 || pSB1A2 || 2079 || 2892 || Amp || Miniprepped/Unconfirmed
+
| BBa_C0051 || c1 represser protein || 750 || pSB1A2 || 2079 || 2892 || Amp || Discontinued
|-
|-
! [[Melbourne/BBa_E0040|P1 5H]]
! [[Melbourne/BBa_E0040|P1 5H]]
-
| BBa_E0040 || GFP || 720 || pSB1A2 || 2079 || 2799 || Amp || Confirmed
+
| BBa_E0040 || GFP || 720 || pSB1A2 || 2079 || 2799 || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_E0430|P1 11A]]
! [[Melbourne/BBa_E0430|P1 11A]]
-
| BBa_E0430 || EYFP(RBS+,LVA-,term) || 878 || pSB1A2 || 2079 || 2957 || Amp || Miniprepped/unconfirmed
+
| BBa_E0430 || EYFP(RBS+,LVA-,term) || 878 || pSB1A2 || 2079 || 2957 || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_R0084|P1 11H]]
! [[Melbourne/BBa_R0084|P1 11H]]
-
| BBa_R0084 || OmpR Promoter || 108 || pSB1A2 || 2079 || 2187 || Amp || Miniprepped/unconfirmed
+
| BBa_R0084 || OmpR Promoter || 108 || pSB1A2 || 2079 || 2187 || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_R0082|P1 15P]]
! [[Melbourne/BBa_R0082|P1 15P]]
-
| BBa_R0082 || OmpR+ Promoter || 108 || pSB1A2 || 2079 || 2187 || Amp || Miniprepped/unconfirmed
+
| BBa_R0082 || OmpR+ Promoter || 108 || pSB1A2 || 2079 || 2187 || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_E0840|P1 16E]]
! [[Melbourne/BBa_E0840|P1 16E]]
-
| BBa_E0840 || RBS,GFP,term || 878 || pSB1A2 || 2079 || 2957 || Amp || Miniprepped/unconfirmed
+
| BBa_E0840 || RBS,GFP,term || 878 || pSB1A2 || 2079 || 2957 || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_R0083|P1 17H]]
! [[Melbourne/BBa_R0083|P1 17H]]
-
| BBa_R0083 || OmpR+ Promoter(truncated version of R0082 || 79 || pSB1A2 || 2079 || 2157 || Amp || Miniprepped/unconfirmed
+
| BBa_R0083 || OmpR+ Promoter(truncated version of R0082 || 79 || pSB1A2 || 2079 || 2157 || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_B0010|P2 3P]]
! [[Melbourne/BBa_B0010|P2 3P]]
-
| BBa_B0010 || Single Terminator || 80 || pSB1A2 || 2079 || 2159 || Amp || Miniprepped/Odd
+
| BBa_B0010 || Single Terminator || 80 || pSB1A2 || 2079 || 2159 || Amp || Discontinued
|-
|-
! [[Melbourne/BBa_Q04510|P2 13K]]
! [[Melbourne/BBa_Q04510|P2 13K]]
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|-
|-
! [[Melbourne/BBa_E0241|P2 15L]]
! [[Melbourne/BBa_E0241|P2 15L]]
-
| BBa_E0241 || PoPs -> GFP || 795 || pSB1A2 || 2079 || 2874 || Amp || Undigested
+
| BBa_E0241 || PoPs -> GFP || 795 || pSB1A2 || 2079 || 2874 || Amp || Discontinued
|-
|-
! [[Melbourne/BBa_I15008|P2 21A]]
! [[Melbourne/BBa_I15008|P2 21A]]
-
| BBa_I15008 || ho1 || 726 || pSB2K3 || 4425 || 5151 || Kan || Miniprepped/Unconfirmed
+
| BBa_I15008 || ho1 || 726 || pSB2K3 || 4425 || 5151 || Kan || Discontinued
|-
|-
! [[Melbourne/BBa_I15009|P2 21C]]
! [[Melbourne/BBa_I15009|P2 21C]]
-
| BBa_I15009 || PcyA || 750 || pSB2K3 || 4425 || 5175 || Kan || Miniprepped/Unconfirmed
+
| BBa_I15009 || PcyA || 750 || pSB2K3 || 4425 || 5175 || Kan || Discontinued
|-
|-
! [[Melbourne/BBa_J61035|P4 8J]]
! [[Melbourne/BBa_J61035|P4 8J]]
-
| BBa_J61035 || EcoRI-GenR-XbaI || 3551 || J61035 || 3551 || 3551 || Amp, Gen || Confirmed
+
| BBa_J61035 || EcoRI-GenR-XbaI || 3551 || J61035 || 3551 || 3551 || Amp, Gen || Glycerol stock in -80
|-
|-
! [[Melbourne/BBa_I15010|I15010]]
! [[Melbourne/BBa_I15010|I15010]]
-
| BBa_I15010 || cph8(cph1/Envz fusion || 2238 || pSB2K3 || 4425 || 6663 || Kan || Miniprepped/Unconfirmed
+
| BBa_I15010 || cph8(cph1/Envz fusion || 2238 || pSB2K3 || 4425 || 6663 || Kan || Discontinued
|-
|-
! [[Melbourne/pJS010|pJS010]]
! [[Melbourne/pJS010|pJS010]]
-
| - || SopII-Htr ||  ||  ||  ||  || Amp || Confirmed
+
| - || SopII-Htr ||  ||  ||  ||  || Amp || Glycerol stock in -80
|-
|-
! [[Melbourne/Fusion|Fusion]]
! [[Melbourne/Fusion|Fusion]]
-
| - || NpHtrII fusion ||  ||  ||  ||  || Amp || Miniprepped/Unconfirmed
+
| - || NpHtrII fusion ||  ||  ||  ||  || Amp || Glycerol stock in -80
|}
|}

Revision as of 07:13, 17 August 2007

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Last Updated: 10/7/07

Parts Used by Melbourne <GRAPHICAL>
Label Registry Part Number Part Name Part Size(bp) Plasmid Vector Plasmid Size(bp) Total size(bp) Resistance Status
P1 1G BBa_B0014 Double Terminator 95 pSB1AK3 3189 3284 Amp, Kan Glycerol stocks in -80
P1 3O BBa_B0034 RBS 12 pSB1A2 2079 2091 Amp Discontinued
P1 5G BBa_C0051 c1 represser protein 750 pSB1A2 2079 2892 Amp Discontinued
P1 5H BBa_E0040 GFP 720 pSB1A2 2079 2799 Amp Glycerol stock in -80
P1 11A BBa_E0430 EYFP(RBS+,LVA-,term) 878 pSB1A2 2079 2957 Amp Glycerol stock in -80
P1 11H BBa_R0084 OmpR Promoter 108 pSB1A2 2079 2187 Amp Glycerol stock in -80
P1 15P BBa_R0082 OmpR+ Promoter 108 pSB1A2 2079 2187 Amp Glycerol stock in -80
P1 16E BBa_E0840 RBS,GFP,term 878 pSB1A2 2079 2957 Amp Glycerol stock in -80
P1 17H BBa_R0083 OmpR+ Promoter(truncated version of R0082 79 pSB1A2 2079 2157 Amp Glycerol stock in -80
P2 3P BBa_B0010 Single Terminator 80 pSB1A2 2079 2159 Amp Discontinued
P2 13K BBa_Q04510 c1 inverter 987 pSB2K3 4425 5412 Kan Transformed
P2 15L BBa_E0241 PoPs -> GFP 795 pSB1A2 2079 2874 Amp Discontinued
P2 21A BBa_I15008 ho1 726 pSB2K3 4425 5151 Kan Discontinued
P2 21C BBa_I15009 PcyA 750 pSB2K3 4425 5175 Kan Discontinued
P4 8J BBa_J61035 EcoRI-GenR-XbaI 3551 J61035 3551 3551 Amp, Gen Glycerol stock in -80
I15010 BBa_I15010 cph8(cph1/Envz fusion 2238 pSB2K3 4425 6663 Kan Discontinued
pJS010 - SopII-Htr Amp Glycerol stock in -80
Fusion - NpHtrII fusion Amp Glycerol stock in -80
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