NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 14: Line 14:
|EcoRI, XbaI
|EcoRI, XbaI
|63 bp
|63 bp
-
|Potential dimer formation! Need to design a new one.
+
|Potential dimer formation!
|-
|-
|-
|-
Line 23: Line 23:
|SpeI, PstI
|SpeI, PstI
|63 bp
|63 bp
-
|Potential dimer formation! Need to design a new one.
+
|Potential dimer formation!
|-
|-
|M_INS_B 5'
|M_INS_B 5'
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
-
|51 bp
+
|52 bp
-
|60.03
+
|60.85
|EcoRI, XbaI
|EcoRI, XbaI
|63 bp
|63 bp
-
|Potential dimer formation! Need to design a new one.
+
|
|-
|-
|-
|-

Revision as of 09:12, 17 August 2007

Name Sequence Length Tm Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTTGCAGTAGTTCTCCAGCT 51 bp EcoRI, XbaI 63 bp Potential dimer formation!
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 55 bp SpeI, PstI 63 bp Potential dimer formation!
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 63 bp
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 55 bp 55.86 SpeI, PstI 90 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1396 bp
RcsC 3'-2 AAGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1396 bp Two extra adenosine was added to the 5' side of the NdeI cutting site.
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Primer_List"