ETHZ/labnotes

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Revision as of 10:53, 18 August 2007

Part/Plasmid Numberings

The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.

[http://partsregistry.org/Part:BBa_B0034 B0034]
[http://partsregistry.org/Part:BBa_R0062 R0062]
[http://partsregistry.org/Part:BBa_R0053 R0053]
[http://partsregistry.org/Part:BBa_J23100 J23100]
[http://partsregistry.org/Part:BBa_J37033 J37033]
[http://partsregistry.org/Part:BBa_E0434 E0434]
[http://partsregistry.org/Part:BBa_B0015 B0015]
[http://partsregistry.org/Part:BBa_Q04400 Q04400]
[http://partsregistry.org/Part:BBa_R0010 R0010]
[http://partsregistry.org/Part:BBa_E0422 E0422]
[http://partsregistry.org/Part:BBa_R0040 R0040]
[http://partsregistry.org/Part:BBa_R0051 R0051]
[http://partsregistry.org/Part:BBa_Q04121 Q04121]
[http://partsregistry.org/Part:BBa_C0053 C0053]
[http://partsregistry.org/Part:BBa_Q04510 Q4510]

Week 1

Date	Completed	People
Mon, 06. Aug. 2007	Preparing the Solutions	Sylke, Raphael, Stefan, Markus, Martin, Christos, Joe
Tue, 07. Aug. 2007	Prepare competent cells for all parts Transformation of all the parts	Sylke, Raphael, Stefan, Markus, Martin, Christos, Joe
Wed, 08. Aug. 2007	Preparing the grown cultures (12) for the MINIPREP (o/n cultures)	Raphael, Stefan
Thu, 09. Aug. 2007	MINIPREP of the grown (10) o/n cultures Gelelectrophoresis of the grown cultures (step: 0.8% Agarose)	Raphael, Stefan, Martin, Christos, Joe
Fri, 10. Aug. 2007	7 working parts/plasmids (step after "DIGESTS"): (B0034, R0062, R0053, E0434, B0015, R0010, E0422) 4 parts/plasmids minipreped: (R0040, R0051, Q04121, C0053)	Christos Markus Stefan
Sat, 11. Aug. 2007	no labwork
Sun, 12. Aug. 2007	labwork cancelled

Week 2

Date	TODO's	Completed	People
Mon, 13. Aug. 2007 start at 3 pm	Prepare competent cells Transformations of J23100, J37033, Q04400, Q04510 Control Restrictions (step after "MINIPREP") R0040, R0051, Q04121, C0053	o/n culture (E.Coli Top10) Control Restrictions (didn't work)	Martin Markus Christos Tim
Tue, 14. Aug. 2007	Morning Shift: Start Preparing competent cells (for J23100, J37033, Q04400, Q04510) Evening Shift: Transformations of J23100, J37033, Q04400, Q04510	Morning Shift: Prepared competent cells (stored in the -80°C freezer in the basement) Evening Shift: Transformation of J23100, J37033, Q04400, Q04510 and R0040, R0051, Q04121, C0053 (in the 37°C incubator until Wednesday) Prepared new Liquid LB, LB Agar (both in the autoclave), Agarose Gel with concentrations of 0.8% and 2.4%	Morning Shift (9am-1pm?): Markus, Tim Evening Shift (5pm-...): Martin, Christos
Wed, 15. Aug. 2007	Ligation (step: "LINK ASSEMBLY"): R0053 + E0422 R0010 + E0422 R0010 + E0434 S/P: R0053, R0010 X/P: E0422, E0434	Ligation didn't work due to bad quality of enzymes (probably)	From 12: Martin, Markus
Thu, 16. Aug. 2007	Miniprep (J23100, J37033, Q04400, Q04510, R0040, R0051, Q04121, C0053) Transformation of #13 and #14	Miniprep of #4 (J23100), #5 (J37033), #8 (Q04400), #11 (R0040), #12 (R0051), #15 (Q04510) One batch is miniprepped (after step 19 in the miniprep procedure) and a second batch is frozen as a backup (which is to be miniprepped from step 3 on) Transformation of #13 (Q04121) and #14 (C0053) Numbers #13 and #14 are now growing in the 37°C oven (step 13 in the transformation procedure)	Markus, Christos, (Martin)
Fri, 17. Aug. 2007	Miniprep of #13 and #14 Check whether miniprep of parts #4 #5 #8 #11 #12 (#13 #14) #15 was successful
Sat, 18. Aug. 2007
Sun, 19. Aug. 2007

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