NYMU Taipei/Primer List
From 2007.igem.org
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|RcsC 3'-2 | |RcsC 3'-2 | ||
| - | |<font color="green"> | + | |<font color="green">GGGGTATAC</font><font color="red">CAACGACTCTTCCATCTTCAC</font> |
|29 bp | |29 bp | ||
|59.63 | |59.63 | ||
Revision as of 11:14, 20 August 2007
| Name | Sequence | Length | Tm (C) | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG | 51 bp | 56.85 | EcoRI, XbaI | 63 bp | Potential dimer formation! |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT | 55 bp | 54.74 | SpeI, PstI | 63 bp | Potential dimer formation! |
| M_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT | 52 bp | 60.85 | EcoRI, XbaI | 90 bp | |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA | 55 bp | 55.86 | SpeI, PstI | 90 bp | |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1396 bp | |
| RcsC 3'-2 | GGGGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1396 bp | Two extra adenosine was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
