NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 30: Line 30:
|60.85
|60.85
|EcoRI, XbaI
|EcoRI, XbaI
-
|90 bp
+
|156 bp
-
|
+
|Potential dimer formation!
|-
|-
|-
|-
Line 39: Line 39:
|55.86
|55.86
|SpeI, PstI
|SpeI, PstI
-
|90 bp
+
|156 bp
 +
|Potential dimer formation!
 +
|-
 +
|-
 +
|H_INS_A 5'
 +
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
 +
|51 bp
 +
|62.45
 +
|EcoRI, XbaI
 +
|129 bp
 +
|Potential dimer formation!
 +
|-
 +
|-
 +
|H_INS_A 3'
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
 +
|56 bp
 +
|57.77
 +
|SpeI, PstI
 +
|129 bp
 +
|Potential dimer formation!
 +
|-
 +
|H_INS_B 5'
 +
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
 +
|51 bp
 +
|57.98
 +
|EcoRI, XbaI
 +
|156 bp
 +
|Potential dimer formation!
 +
|-
 +
|-
 +
|H_INS_B 3'
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
 +
|55 bp
 +
|55.10
 +
|SpeI, PstI
 +
|156 bp
|
|
|-
|-

Revision as of 09:10, 21 August 2007

Name Sequence Length Tm (C) Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG 51 bp 56.85 EcoRI, XbaI 63 bp Potential dimer formation!
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 55 bp 54.74 SpeI, PstI 63 bp Potential dimer formation!
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 156 bp Potential dimer formation!
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 55 bp 55.86 SpeI, PstI 156 bp Potential dimer formation!
H_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG 51 bp 62.45 EcoRI, XbaI 129 bp Potential dimer formation!
H_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 56 bp 57.77 SpeI, PstI 129 bp Potential dimer formation!
H_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG 51 bp 57.98 EcoRI, XbaI 156 bp Potential dimer formation!
H_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 55 bp 55.10 SpeI, PstI 156 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1404 bp
RcsC 3'-2 GGGGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1404 bp Three extra guanosine was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
  • [http://www.mdbio.com.tw/pfu.htm pfu primer]
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