NYMU Taipei/Lab Notes/2007 8 27
From 2007.igem.org
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*Enzyme Digestion | *Enzyme Digestion | ||
**pOmpC: 14ul EYFP: 3ul | **pOmpC: 14ul EYFP: 3ul | ||
| + | **RE: 1ul | ||
**Heat inactivation: 65°C 20min. | **Heat inactivation: 65°C 20min. | ||
*Culture tar-EnvZ, pLacIQ in 5 ml LB liquid medium (Kan:20ug/ml) | *Culture tar-EnvZ, pLacIQ in 5 ml LB liquid medium (Kan:20ug/ml) | ||
Revision as of 09:21, 27 August 2007
- plasmid concentration after liquid culture extraction
| part | concentration (ug/ul) |
|---|---|
| pOmpC | 6 |
| EYFP | 30 |
| ECFP | 23 |
| CinR | 15 |
| HSL | 49 |
| pCinHSL | 7 |
| pCinHSLRBS | 12 |
| RBS | 14 |
| D-term | 27 |
- Ligation
- Insert:vector=3:1
- Vector: 300ng/ul(2ul pOmpC after enzyme digestion)
- Insert: 100ng/ul(2ul EYFP after enzyme digestion)
- Ligase: 1ul
- Final volume: 10ul
- Enzyme Digestion
- pOmpC: 14ul EYFP: 3ul
- RE: 1ul
- Heat inactivation: 65°C 20min.
- Culture tar-EnvZ, pLacIQ in 5 ml LB liquid medium (Kan:20ug/ml)
