Week 5
From 2007.igem.org
(Difference between revisions)
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::'''08/01/07''' | ::'''08/01/07''' | ||
| + | *We get along with: | ||
| + | -Miniprep of I52034 and B0015; | ||
| + | -Digestion of B0015 with Xba/Pst1 | ||
| + | I52034 with Spe/Pst1; | ||
| + | -extraction from gel (we can’t see B0015); | ||
| + | -R0015+I13507 (I763007) ligation which doesn’t give colonies so we transform with the other 10 ul of ligation; | ||
| + | -J04500+C0051 (I763005) ligation which gives colonies so we inoculate in 5ml; | ||
| + | -transformation for C0012 (4ul) and for J22101 (2ul); | ||
| + | -a new R0051+ I13507 (I763007) (vector 4ul, insert 8ul) ligation. | ||
Revision as of 12:25, 27 August 2007
- 07/30/07
- We control bacterial grew (link).
- Transformation for C0012 and J52034 and B0015.
- We get along with the ligation R0051+I13507 (I763007) (vector 2 ul, insert 6 ul) and the ligation J04500+ C0051 (I763005) (vector 2 ul, insert 10 ul).
- 07/31/07
- There isn’t any colony for C0012, so we inoculate I52034 and B0015 colony in 5 ml.
- We plate 30/07 ligations.
- We get along with the J04431 fluorescence test:
-From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation. -We verify the OD and the fluorescence every 15 minutes. The fluorescence compare at the istance t=210min and at the OD=0.5.
- At the end of this test we decide to repeat it to see if we can switch off the fluorescent cells with a major quantity of glucose to confirm our assumptions.
We will attend this test on 07/08.
- 08/01/07
- We get along with:
-Miniprep of I52034 and B0015; -Digestion of B0015 with Xba/Pst1
I52034 with Spe/Pst1;
-extraction from gel (we can’t see B0015); -R0015+I13507 (I763007) ligation which doesn’t give colonies so we transform with the other 10 ul of ligation; -J04500+C0051 (I763005) ligation which gives colonies so we inoculate in 5ml; -transformation for C0012 (4ul) and for J22101 (2ul); -a new R0051+ I13507 (I763007) (vector 4ul, insert 8ul) ligation.
- 08/02/07
- 08/03/07
