McGill
From 2007.igem.org
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This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that revesibly associate.<br> | This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that revesibly associate.<br> | ||
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Our project consists of a two-gene oscillator with coupling by an Autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations at different cell densities. | Our project consists of a two-gene oscillator with coupling by an Autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations at different cell densities. | ||
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| + | [[What it's all about]] <br> | ||
| + | [[Background Papers]] <br> | ||
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Revision as of 13:43, 18 May 2007
Contents |
Project Overviews
Team 1 - Fluorescence Complimentation
This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that revesibly associate.
What it's all about
Background Papers
Team 2 - Repressilator
Our project consists of a two-gene oscillator with coupling by an Autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations at different cell densities.
What it's all about
Background Papers
In the Lab
The Team
Important Notes
When you make the links for the wiki pages, please follow this example:
[[McGill/Link_to_page|Display Name]]
An actual link would look like this in wiki code: [[McGill/Team_1:_Fluorescence_Complementation|Team 1: Fluorescence Complementation]]
Thanks! - Hanmo
