NYMU Taipei/Lab Notes/2007 8 30

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  • Enzyme Digestion (RBS)
    • pOmpC: 11ul
    • SpeI: 1ul
    • PstI: 1ul
    • 10x buffer: 2, 2ul
    • 10x BSA: 2ul
    • H2O: 3ul
    • Total: 20ul
    • 37°C, 2h
    • Heat inactivation: 65°C 20min
  • Enzyme Digestion (cinR)
    • cinR: 11ul
    • XbaI: 1ul
    • PstI: 1ul
    • 10x buffer: 3, 2ul
    • 10x BSA: 2ul
    • H2O: 3ul
    • Total: 20ul
    • 37°C, 2h
    • Heat inactivation: 65°C 20min
  • Enzyme Digestion (HSL)
    • HSL: 3ul
    • XbaI: 1ul
    • PstI: 1ul
    • 10x buffer: 3, 2ul
    • 10x BSA: 2ul
    • H2O: 11ul
    • Total: 20ul
    • 37°C, 2h
    • Heat inactivation: 65°C 20min
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