From 2007.igem.org

Revision as of 05:13, 30 August 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Revision as of 05:20, 30 August 2007 (view source) Lihsiangyen (Talk | contribs) Newer edit →
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	**Heat inactivation: 65°C 20min		**Heat inactivation: 65°C 20min
	*Enzyme Digestion (HSL)		*Enzyme Digestion (HSL)
-	**HSL: 3ul	+	**HSL: 4ul
	**XbaI: 1ul		**XbaI: 1ul
	**PstI: 1ul		**PstI: 1ul
	**10x buffer: 3, 2ul		**10x buffer: 3, 2ul
	**10x BSA: 2ul		**10x BSA: 2ul
-	**H2O: 11ul	+	**H2O: 10ul
	**Total: 20ul		**Total: 20ul
	**37°C, 2h		**37°C, 2h
	**Heat inactivation: 65°C 20min		**Heat inactivation: 65°C 20min

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Revision as of 05:20, 30 August 2007

• Enzyme Digestion (RBS)
  • pOmpC: 11ul
  • SpeI: 1ul
  • PstI: 1ul
  • 10x buffer: 2, 2ul
  • 10x BSA: 2ul
  • H2O: 3ul
  • Total: 20ul
  • 37°C, 2h
  • Heat inactivation: 65°C 20min
• Enzyme Digestion (cinR)
  • cinR: 11ul
  • XbaI: 1ul
  • PstI: 1ul
  • 10x buffer: 3, 2ul
  • 10x BSA: 2ul
  • H2O: 3ul
  • Total: 20ul
  • 37°C, 2h
  • Heat inactivation: 65°C 20min
• Enzyme Digestion (HSL)
  • HSL: 4ul
  • XbaI: 1ul
  • PstI: 1ul
  • 10x buffer: 3, 2ul
  • 10x BSA: 2ul
  • H2O: 10ul
  • Total: 20ul
  • 37°C, 2h
  • Heat inactivation: 65°C 20min