NYMU Taipei/Lab Notes/2007 9 2
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
| Line 1: | Line 1: | ||
| - | *Ligation of (RBS+cinR+RBS+HSL): 4(?) | + | *Ligation of (RBS+cinR+RBS+HSL): 4(?) clones |
*Ligation of (RBS+cinR ver. 2): multiple(?) colonies | *Ligation of (RBS+cinR ver. 2): multiple(?) colonies | ||
| - | + | *Transfer each to 4 5ml LB liquid culture medium with 1 clone respectly. | |
| - | *Transfer each to 4 LB liquid culture | + | |
**(RBS+cinR+RBS+HSL)1,(RBS+cinR+RBS+HSL)2,(RBS+cinR+RBS+HSL)3,(RBS+cinR+RBS+HSL)4 | **(RBS+cinR+RBS+HSL)1,(RBS+cinR+RBS+HSL)2,(RBS+cinR+RBS+HSL)3,(RBS+cinR+RBS+HSL)4 | ||
**(RBS+cinR ver. 2)1,(RBS+cinR ver. 2)2,(RBS+cinR ver. 2)3,(RBS+cinR ver. 2)4 | **(RBS+cinR ver. 2)1,(RBS+cinR ver. 2)2,(RBS+cinR ver. 2)3,(RBS+cinR ver. 2)4 | ||
*Culture at 37C for 8 hours | *Culture at 37C for 8 hours | ||
*Store at 4C | *Store at 4C | ||
| - | |||
*Rearrange of our parts in the box "IGEM 2007 parts" | *Rearrange of our parts in the box "IGEM 2007 parts" | ||
| + | ---- | ||
| + | ==Who attend?== | ||
| + | * 威,惇茹,穎駿,志賢 | ||
Latest revision as of 03:08, 5 September 2007
- Ligation of (RBS+cinR+RBS+HSL): 4(?) clones
- Ligation of (RBS+cinR ver. 2): multiple(?) colonies
- Transfer each to 4 5ml LB liquid culture medium with 1 clone respectly.
- (RBS+cinR+RBS+HSL)1,(RBS+cinR+RBS+HSL)2,(RBS+cinR+RBS+HSL)3,(RBS+cinR+RBS+HSL)4
- (RBS+cinR ver. 2)1,(RBS+cinR ver. 2)2,(RBS+cinR ver. 2)3,(RBS+cinR ver. 2)4
- Culture at 37C for 8 hours
- Store at 4C
- Rearrange of our parts in the box "IGEM 2007 parts"
Who attend?
- 威,惇茹,穎駿,志賢
