Alberta/Calender/September
From 2007.igem.org
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To Do list for tomorrow | To Do list for tomorrow | ||
| - | - Restriction on DB needs to be done | + | - Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br> |
| + | |||
| + | -complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br> | ||
- If I0500 comes in from calgary it needs to be transformed. | - If I0500 comes in from calgary it needs to be transformed. | ||
| - | - Transform | + | - Transform R0080 which is a back up promoter just in case? |
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| - | |||
| - | -Note we also have to do sequenceing on BB and BE | + | -Note we also have to do sequenceing on BB and BE and DB |
Shout out to calgary for saving our necks | Shout out to calgary for saving our necks | ||
Revision as of 04:12, 5 September 2007
September
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To August 2007
Back to UofA iGEM Home
== September 1 ==
Lab Notes
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later
-CZ
September 2
September 3
September 4
Schedule:
JP,ML,JG 7:00pm
For the record:
Buddy = BuOH DH 1235 bp
Betty = Bb-bhBu-coa dh 923 bp
Benny = Butyryl coa dh 1184 bp
enny = Enoyl coa Hydratase 860 bp
Diezel Blaze = buAld-Dh 2639 bp
Notes:
Moving was done this mid-day.
New location M349
JG has key & access
- MC, ED, JG
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC
Night Crew
-JP, JG
Set up new lab
Things we need - Water Bath, Scale, disposable 13ml overnight tubes, Tip waste
lab work
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab
To Do list for tomorrow
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished!
- If I0500 comes in from calgary it needs to be transformed.
- Transform R0080 which is a back up promoter just in case?
-Note we also have to do sequenceing on BB and BE and DB
Shout out to calgary for saving our necks Shout out to the move in crew to the top
September 5
Schedule:
NG,JP,ML 7:00pm
MC will be in AM (JG - what time will you be in because you have the key?) thnx - MC
JG can get up as early as you would like lets say 8:00 (if you are willing to get up earlier just let me know)
September 6
Schedule:
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)
September 7
Schedule:
MC, JG 8:00 AM ... If you would like to meet later michelle just let me know i am free untill 11:00am But i only have one lecture that day any time else is good
I don't have class all day - so whenever is good for you - MC
September 8
Schedule:
NG AL JP Noon?
I'm good to go at 1:15pm -jp
I can be there at 11am - al
September 9
Schedule:
JG ED NG Noon?
September 10
Schedule:
September 11
September 12
September 13
September 14
September 15
September 16
September 17
September 18
September 19
September 20
September 21
September 22
September 23
September 24
September 25
September 26
September 27
September 28
September 29
September 30
