From 2007.igem.org

Revision as of 10:23, 10 September 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Latest revision as of 10:30, 10 September 2007 (view source) Lihsiangyen (Talk | contribs)
Line 6:		Line 6:
	**ddH2O:5ul		**ddH2O:5ul
	**total: 10ul		**total: 10ul
		+	*ligation time:1hr30min
	*Transformation		*Transformation
	**ligated DNA:2ul		**ligated DNA:2ul

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Latest revision as of 10:30, 10 September 2007

• Ligation(H_INS_A)
  • H_INS_A1:1ul
  • pOmpC:2ul
  • 10X ligase:1 ul
  • 10X buffer:1 ul
  • ddH2O:5ul
  • total: 10ul
• ligation time:1hr30min
• Transformation
  • ligated DNA:2ul
  • compenent cell:100ul
• apply the cells on the agar plate

• plate culture failed!
• possibility:concentration of H_INS_A1 is not high enough