Vector choice
From 2007.igem.org
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Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb. | Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb. | ||
[[Image:YCplac22_map.jpg]] | [[Image:YCplac22_map.jpg]] | ||
| + | |||
| + | Selected features | ||
| + | |||
| + | lac_promoter 142 - 171 | ||
| + | M13_pUC_rev_primer 185 - 207 | ||
| + | M13_reverse_primer 206 - 224 | ||
| + | M13_forward20_primer 306 - 290 | ||
| + | M13_pUC_fwd_primer 321 - 299 | ||
| + | CEN4 504 - 1356 | ||
| + | Orf frame 2 2740 - 2066 | ||
| + | TRP1 2740 - 2066 | ||
| + | AmpR_promoter 2983 - 3011 | ||
| + | Orf frame 2 3053 - 3913 | ||
| + | Ampicillin 3053 - 3913 | ||
| + | pBR322_origin 4068 - 4687 | ||
| + | |||
| + | Unique restriction sites | ||
| + | HindIII 233 | ||
| + | PstI 249 | ||
| + | SalI 251 | ||
| + | XbaI 257 | ||
| + | BamHI 263 | ||
| + | SmaI 270 | ||
| + | KpnI 276 | ||
| + | SacI 282 | ||
| + | EcoRI 284 | ||
| + | NarI 446 | ||
| + | SpeI 550 | ||
| + | NheI 1801 | ||
| + | BglII 1986 | ||
| + | EcoRV 2455 | ||
| + | PmlI 2780 | ||
| + | AatII 2921 | ||
Revision as of 19:50, 10 September 2007
Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb.
Selected features
lac_promoter 142 - 171 M13_pUC_rev_primer 185 - 207 M13_reverse_primer 206 - 224 M13_forward20_primer 306 - 290 M13_pUC_fwd_primer 321 - 299 CEN4 504 - 1356 Orf frame 2 2740 - 2066 TRP1 2740 - 2066 AmpR_promoter 2983 - 3011 Orf frame 2 3053 - 3913 Ampicillin 3053 - 3913 pBR322_origin 4068 - 4687
Unique restriction sites HindIII 233 PstI 249 SalI 251 XbaI 257 BamHI 263 SmaI 270 KpnI 276 SacI 282 EcoRI 284 NarI 446 SpeI 550 NheI 1801 BglII 1986 EcoRV 2455 PmlI 2780 AatII 2921
