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Revision as of 19:50, 10 September 2007 (view source) Robertina (Talk | contribs) ← Older edit		Revision as of 19:51, 10 September 2007 (view source) Robertina (Talk | contribs) Newer edit →
Line 5:		Line 5:
	lac_promoter        142 - 171		lac_promoter        142 - 171
-	M13_pUC_rev_primer  185 - 207	+
		+	M13_pUC_rev_primer  185 - 207
		+
	M13_reverse_primer  206 - 224		M13_reverse_primer  206 - 224
		+
	M13_forward20_primer 306 - 290		M13_forward20_primer 306 - 290
-	M13_pUC_fwd_primer  321 - 299	+
-	CEN4                504 - 1356	+	M13_pUC_fwd_primer  321 - 299
		+
		+	CEN4                504 - 1356
		+
	Orf frame 2          2740 - 2066		Orf frame 2          2740 - 2066
		+
	TRP1                2740 - 2066		TRP1                2740 - 2066
		+
	AmpR_promoter        2983 - 3011		AmpR_promoter        2983 - 3011
		+
	Orf frame 2          3053 - 3913		Orf frame 2          3053 - 3913
-	Ampicillin          3053 - 3913	+
		+	Ampicillin          3053 - 3913
		+
	pBR322_origin        4068 - 4687		pBR322_origin        4068 - 4687
		+
	Unique restriction sites		Unique restriction sites
-	HindIII  233	+	HindIII  233
		+
	PstI  249		PstI  249
	SalI  251		SalI  251

---

Revision as of 19:51, 10 September 2007

Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb.

Selected features

lac_promoter 142 - 171

M13_pUC_rev_primer 185 - 207

M13_reverse_primer 206 - 224

M13_forward20_primer 306 - 290

M13_pUC_fwd_primer 321 - 299

CEN4 504 - 1356

Orf frame 2 2740 - 2066

TRP1 2740 - 2066

AmpR_promoter 2983 - 3011

Orf frame 2 3053 - 3913

Ampicillin 3053 - 3913

pBR322_origin 4068 - 4687

Unique restriction sites HindIII 233

PstI 249 SalI 251 XbaI 257 BamHI 263 SmaI 270 KpnI 276 SacI 282 EcoRI 284 NarI 446 SpeI 550 NheI 1801 BglII 1986 EcoRV 2455 PmlI 2780 AatII 2921