NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 50: Line 50:
|Potential dimer formation!
|Potential dimer formation!
|-
|-
 +
|TAT F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">ATGAACAATAACGATCTCTTTCAGG</font>
 +
|56 bp
 +
|59.92
 +
|XbaI
 +
| bp
 +
|XbaI+RBS
|-
|-
|H_INS_A F
|H_INS_A F
-
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
+
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|54 bp
|54 bp
|62.45
|62.45
|XbaI
|XbaI
|122 bp
|122 bp
-
|Potential dimer formation! XbaI+RBS
+
|Potential dimer formation! XbaI+RBS, ATG removed.
|-
|-
 +
|TAT signal H_INS_A R
 +
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
 +
|32 bp
 +
|59.26
 +
|
 +
|bp
 +
|Potential dimer formation!
|-
|-
|H_INS_A R'
|H_INS_A R'

Revision as of 09:49, 12 September 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 56 bp 59.92 XbaI bp XbaI+RBS
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS, ATG removed.
TAT signal H_INS_A R CAGCATTGTTCCACAATGCCCGCTTGCGCCGC 32 bp 59.26 bp Potential dimer formation!
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
M_IDE F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCGGAACGGGCTCGTGTGGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP3 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP7 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
  • [http://www.mdbio.com.tw/pfu.htm pfu primer]
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