NYMU Taipei/Lab Notes/2007 9 13
From 2007.igem.org
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*** the best temperature should be around 55.5 degree | *** the best temperature should be around 55.5 degree | ||
* PCR product concentration summary | * PCR product concentration summary | ||
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Revision as of 13:08, 13 September 2007
- PCR issue
- We tested our PCR product (5uL) from different annealing temperature via GEL
- From imageJ comparison, the concentration of product rise with temperature before 60 degree
- GEL (left to right: 49.8, 52.3, 55.5, 58.7, 60.9 degree)
-
- imageJ
-
- marker (100 bp, 40ng/10uL = 4ng/uL)
- the best temperature should be around 55.5 degree
- PCR product concentration summary
| name | product | dulition | A260 | A260/A280 | A260/A230 |
|---|---|---|---|---|---|
| 9/5 海威 | INS_A #1 (A1) | 6uL + 594uL ddH2O | |||
| 9/5 海威 | INS_A #2 (A2) | 6uL + 594uL ddH2O | |||
| 9/5 海威 | INS_B #1 (B1) | 6uL + 594uL ddH2O | |||
| 9/5 海威 | INS_B #2 (B2) | 6uL + 594uL ddH2O | |||
| 9/12 德銘 | INS_A #1 (A1, 49.8) | 6uL + 594uL ddH2O | |||
| 9/12 德銘 | INS_A #2 (A2, 52.3) | 6uL + 594uL ddH2O | |||
| 9/12 德銘 | INS_A #3 (A3, 55.5) | 6uL + 594uL ddH2O | |||
| 9/12 德銘 | INS_A #4 (A4, 58.7) | 6uL + 594uL ddH2O | |||
| 9/12 德銘 | INS_A #5 (A1, 60.9) | 6uL + 594uL ddH2O |
