Paris/September 13
From 2007.igem.org
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|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D57 | | style="background: #ccffcc;" |D57 | ||
| - | |lox71- | + | |lox71-B0015(T) BI |
| - | |L31.1, L31.3, L31.4 lox71- | + | |L31.1, L31.3, L31.4 lox71-B0015(T) <bbpart>pSB1A2</bbpart> |
|XbaI | |XbaI | ||
|Pst1 | |Pst1 | ||
| | | | ||
| - | |lox71- | + | |lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart> |
| | | | ||
| | | | ||
| Line 55: | Line 55: | ||
|pTet>>lox71-gfptripart BI | |pTet>>lox71-gfptripart BI | ||
|L34.1, L34.2, L34.3 pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | |L34.1, L34.2, L34.3 pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | ||
| - | | | + | |XbaI |
|PstI | |PstI | ||
| | | | ||
| Line 66: | Line 66: | ||
|araC/pBad>>lox71-gfptripart BI | |araC/pBad>>lox71-gfptripart BI | ||
|L35.1, L35.2 & L35.3 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | |L35.1, L35.2 & L35.3 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart> | ||
| - | | | + | |XbaI |
|PstI | |PstI | ||
| | | | ||
Revision as of 19:44, 13 September 2007
Digestions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D56 | araC/pBad>>RBS-Cre BBinsert | L26.1 araC/pbad>>RBS-Cre in J61002 | EcoRI | Pst1 | araC/pBad>>RBS-Cre BBinsert (for transfer to the low-copy plasmid vector) | ||||
| D57 | lox71-B0015(T) BI | L31.1, L31.3, L31.4 lox71-B0015(T) pSB1A2 | XbaI | Pst1 | lox71-B0015(T) extracted for use as a BI pSB1A2 | ||||
| D58 | B0015(T)-lox66 BV | L32.1, L32.2, L32.3 B0015(T)-lox66 BV | SpeI | PstI | B0015(T)-lox66 for use as a BV pSB1A2 | ||||
| D59 | pTet>>lox71-gfptripart BI | L34.1, L34.2, L34.3 pTet>>lox71-gfptripart BBa_J61002 | XbaI | PstI | pTet>>lox71-gfptripart for use as a BI | ||||
| D60 | araC/pBad>>lox71-gfptripart BI | L35.1, L35.2 & L35.3 araC/pBad>>lox71-gfptripart BBa_J61002 | XbaI | PstI | araC/pBad>>lox71-gfptripart for use as a BI | ||||
| D61 | lox66-mRFP FV | L36.1, L36.2 & L36.3 lox66-mRFP | EcoRI | XbaI | lox66-gfptri part (strongRBS-ORF-T) ready for use as a forward vector | ||||
| D62 | lox66-RBSDapAcoli BI | L37.1, L37.2 & L37.3 lox66-RBSDapAcoli | XbaI | PstI | lox66-RBSDapAcoli ready for use as a Backward insert | ||||
| D63 | RBSDapAcoli BI | L39.1, L39.2 & L39.3 RBSDapAcoli | XbaI | PstI | RBSDapAcoli BI (for insertion behind T-lox66) | ||||
| D64 | FRT-CmR-FRT BBa_J61025 BV | MP14.1 BBa_J61025 | SpeI | PstI | FRT-CmR-FRT BBa_J61025 (for backward introduction of a casette for Wanner K insertion) | ||||
| D65 | FRT-CmR-FRT BBa_J61025 FV | MP14.1 BBa_J61025 | EcoR | XbaI | FRT-CmR-FRT BBa_J61025 (for forward introduction of a casette for Wanner K insertion) | ||||
| D65 | KanR BBa_J61025 FV | MP12.1 BBa_P1003 in pSB1A2 | EcoR | XbaI | KanR BBa_J61025 (for forward introduction of a casette for Wanner K insertion) | ||||
| D66 | RBS-DapAsubtilis BI | L43.1, L43.2 & L43.3 RBS-DapAsubtilis pSB1A2 | XbaI | PstI | RBS-DapAsubtilis for use as a BI | ||||
| D67 | attB BV | MP15.1 | SpeI | PstI | vector containing attB for use as a BV | ||||
| D68 | gfptripart-lox66 BV | L29.1 gfptripart-lox66 pSB1A2 | SpeI | PstI | |||||
digestion mix:
- 25µl plasmid
- 1µl BSA
- 10 µl NEB2
- 60µl H2O
- 2µl Enzyme1
- 2µl Enzyme2
