Melbourne/meeting 7 September 2007
From 2007.igem.org
(Difference between revisions)
Revision as of 05:56, 14 September 2007
Contents |
5:30 Action points from last meeting:
- Pat & Alisa: LacZ reporter plan - complete
- Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!
- Phil: re prism :Edmund optics:VIS60 Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)
N47-279 eqilateral prism 35mm long 35mm face SGD$162
- Phil: Chase Status of #BBa-M30109 & #BBa-V1012 - Arrived
- Craig: Alkaline lysis technique for miniprep to be documented
- Craig: Protein extraction technique –done sent to Phil by email.
- Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains: Alisa will set up plates for Daisy to work from by monday so we will have a store of competant cells by Tues/Wed
- Chris: Gas vesicle expression experiment by 21/8/07.-Done
5:35 Status Reports: (5 minutes each including any discussion)
- Alisa: Ligation experiments progress
- Craig ligated the ompR promoter to the RBS-LacZ-Ter and c1 inverter, confirmation digest and gel to be done on saturday and parts sent for sequencing confirmation on Monday.
- Additional ligations to be set up and confirmed during the week: OmpR test harness(Ompr-RBS-LacZ-ter) to be ligated behind M30109, Ompr-c1 inverter to be ligated behind M30109.
- Pat & Alisa: Red light receptor progress/design/plan- needs?
- these include to above ligations. We need to work out how we are going to test these parts
- Jan: Blue light receptor design/plan progress - needs? (Absent from meeting)
- Phil: Gas vesicles progress/design/plan - needs?
- Progress good, now on round 3 of mutagenesis (3 plates x2).
- Need miniprep of six colonies on Monday and Digest with PstI, I will pick colonies Sunday.
- Buoyancy experiment confused re IPTG, -IPTG,+IPTG but definitely differences. See attached photos at 1hour,6hours,18hours,96hours and last 3 at 126hours
- Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
5.55 New Agenda Items:
- Phil: Administrative Timetable: (8 Weeks to go)
- Final Team roster and Jamboree attendance fees due. Who is going? Jan & Phil perhaps, others??? Need to find out state of funds so that we can work out how many people we are able to send
- Chris: Next years Comp Plan/preparations - same state as last week
- Any other business
- Next meeting: <b/> 28th August (Friday) 5.30PM.
6:15 End
Igem Administrative Timetable:
| 1 September | Final team rosters due. |
| 1 September | Jamboree attendance fees due $100USD per team member. |
| 26 October | Project and part documentation due DNA received USA. |
| 26 October | BioBrick Part DNA received by the Registry. |
| 3-4 November | iGEM Competition Jamboree, MIT, USA. |
.
Documentation wiki status:
- Priority should be on plans and experimental report analysis and conclusions-----Alisa .
- If only Pat & Alisa doing lab work not much documentation needed.
- Equipement:
- MissingLas3000 method (in lab notebook)
- Missing DNA measurement protocol (eppendorf)
- Reagents pages incomplete:
- Some location pictures not finished or uploaded.
- Some newer reagents not entered.
- Secondary reagents pages not made:
- LB, Plates, TAE , NZY+ Broth
- Protocol part:
- No connections from Protocols to reagents.
- Ligation protocol missing
- Lab notebook:
- Format uses too many lines to see connections between days.
- Latter part missing links
- Can table of contents be restricted to date level?
- Experimental reports:
- Lacks discussion/conclusion
- Ligation experiment missing
- Lattest few gells not marked up and entered.
- Project overview link broken
- Background:
- GFP missing ,
- Red photo sensor to GFP test harness missing.
- Plan: Only Gas vesicles plan entered.
| Individual | Desired time committment |
|---|---|
| Pat | About 4h/wk Monday afternoon or Tuesday morning |
| Alisa | About 4h/wk - Thursday afternoon |
| Jan | About 10h/pw - flexible weekday mornings |
| Phil | About 18h/pw - Thursday morning, Friday, Saturday or Sunday, |
