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Revision as of 14:26, 12 September 2007 (view source) Davidpz (Talk | contribs) ← Older edit		Revision as of 14:34, 14 September 2007 (view source) Davidpz (Talk | contribs) (→DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR) Newer edit →
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	- Primer2: 34: 2.5µL (final 0.5µM)<br>		- Primer2: 34: 2.5µL (final 0.5µM)<br>
-	[[Image:WannerDapA09122007.jpg|right|100px]]	+	[[Image:WannerDapA09122007.jpg|right|300px]]
	* PCR Program:<br>		* PCR Program:<br>

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Revision as of 14:34, 14 September 2007

DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR

• Material:
  

- pKD3/pKD4: 1 µL (30ng/60ng)
- Tampon: 10µL
- dNTP: 1µL (final 200µmol each)
- Taq: 0.5µL
- Water: 32.5µL
- Primer1: 33: 2.5µL (final 0.5µM)
- Primer2: 34: 2.5µL (final 0.5µM)

• PCR Program:
  

35 cycles:
- 95°C: 5min
- 95°C: 30s (X35)
- 55°C: 30s (X35)
- 72°C: 1min30 (X35)
- 72°C: 10 min
- 4°C: inf

• Quantity: ng/µL
  
• Expected size: bp

• After purification:
  

2µL of purification product (2µL of ladder)