Chiba/Week4

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FliC Group AHL Group
  • Swaming test
  1. FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation（Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの）
  2. LB培地で37℃12h2ml培養
  3. トリプトン培地（Agar0.25%）の中央に1μinoculate
  4. 30℃、24-48h,Taxis
  5. Taxis ling を観察
  • Sample

Swam4-2-2.jpg

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