NYMU Taipei/Lab Notes/2007 9 16

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  • Enzyme digestion: CinR+HSL (with RBS) with EcoRI, SpeI
    • CinR+HSL (1~4): 14ul
    • EcoRI: 1ul (20,000 units/ml)
    • SpeI: 1ul (10,000 units/ml)
    • 10x EcoRI buffer: 2ul
    • Total: 20ul
  • Enzyme digestion: D-term with EcoRI
    • D-term: 11ul
    • EcoRI: 1ul
    • 10x EcoRI buffer: 2ul
    • H2O: 6ul
    • Total: 20ul
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