NYMU Taipei/phptest
From 2007.igem.org
(Difference between revisions)
| Line 19: | Line 19: | ||
<INPUT type="text" name="SI" > size (bp) of insert part (SI)<BR> | <INPUT type="text" name="SI" > size (bp) of insert part (SI)<BR> | ||
<INPUT type="text" name="SV" > size (bp) of vector part (SV)<BR> | <INPUT type="text" name="SV" > size (bp) of vector part (SV)<BR> | ||
| - | <INPUT type="text" name=" | + | <INPUT type="text" name="CI" > concentration (ug/uL) of insert (CI)<BR> |
| - | <INPUT type="text" name=" | + | <INPUT type="text" name="CV" > concentration (ug/uL) of vector (CV)<BR> |
| - | <INPUT type="text" name=" | + | <INPUT type="text" name="VI" > volume (uL) of insert for gel separation (VI)<BR> |
<INPUT type="text" name="L" > volume (uL) of insert for ligation (L)<BR> | <INPUT type="text" name="L" > volume (uL) of insert for ligation (L)<BR> | ||
<input type=button value="送出" Onclick="CheckId(SI.value, SV.value);"> | <input type=button value="送出" Onclick="CheckId(SI.value, SV.value);"> | ||
| Line 32: | Line 32: | ||
** 10X buffer (2uL) | ** 10X buffer (2uL) | ||
** BSA (2uL) | ** BSA (2uL) | ||
| - | ** plasmid + ddH2O (14uL) | + | ** plasmid + ddH2O (14uL = VI + water, 1 <= VI <= 14) |
---- | ---- | ||
* total gel separation volume is 50 uL | * total gel separation volume is 50 uL | ||
Revision as of 07:03, 16 September 2007
- total digestion volume is 20 uL (typical)
- enzyme 1 and enzyme 2 (1uL * 2)
- 10X buffer (2uL)
- BSA (2uL)
- plasmid + ddH2O (14uL = VI + water, 1 <= VI <= 14)
- total gel separation volume is 50 uL
- vector weight is 300 ng
- formula
- (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
- (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
- VV (uL) is volume of vector
- CV (ng/uL) is concentration of vector
