Bangalore
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To investigate multistability and hysteresis in combinatorially constructed synthetic Vibrio quorum sensing circuits | To investigate multistability and hysteresis in combinatorially constructed synthetic Vibrio quorum sensing circuits | ||
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| + | == The Target - Vibrio Quorum Sensing System == | ||
Quorum sensing is a phenomenon by which bacteria sense a critical cell density before turning on the expression of certain genes. It involves the gradual build-up of a chemical termed the 'autoinducer' in the cell. The autoinducer freely diffuses across the cell membrane and hence, its concentration is population density dependent. When the concentration crosses a threshold, the bacteria switch to a different physiological state such as bioluminescence, virulent gene expression, and bio-film formation. | Quorum sensing is a phenomenon by which bacteria sense a critical cell density before turning on the expression of certain genes. It involves the gradual build-up of a chemical termed the 'autoinducer' in the cell. The autoinducer freely diffuses across the cell membrane and hence, its concentration is population density dependent. When the concentration crosses a threshold, the bacteria switch to a different physiological state such as bioluminescence, virulent gene expression, and bio-film formation. | ||
In Vibrio fischeri, when the population density (and hence the concentration of autoinducer) crosses a certain threshold, the expression of a set of genes that is required for bioluminescence is turned on. The production of the autoinducer is under the control of a gene, the expression of which involves positive feedback. The following figure shows the various components of this system (Ref. 1). | In Vibrio fischeri, when the population density (and hence the concentration of autoinducer) crosses a certain threshold, the expression of a set of genes that is required for bioluminescence is turned on. The production of the autoinducer is under the control of a gene, the expression of which involves positive feedback. The following figure shows the various components of this system (Ref. 1). | ||
Revision as of 07:31, 29 May 2007
The Company
- Mukund: Chief Mentor, Consultant and The Godfather
- Sugat: Right-hand Man and Manager of Operations
- Nilesh: The Man for all Seasons
- Vivek: Chief Troubleshooter, often his role is confused with one of a Troublemaker
- Krishna: The Sharpshooter
- Senthil: The Wikiman, also fondly referred to as the "Bio Info God"
- Varun: The Voice of Reason and Anti-Reason
- K12Z1: The Workhorse
The Mission
To investigate multistability and hysteresis in combinatorially constructed synthetic Vibrio quorum sensing circuits
The Target - Vibrio Quorum Sensing System
Quorum sensing is a phenomenon by which bacteria sense a critical cell density before turning on the expression of certain genes. It involves the gradual build-up of a chemical termed the 'autoinducer' in the cell. The autoinducer freely diffuses across the cell membrane and hence, its concentration is population density dependent. When the concentration crosses a threshold, the bacteria switch to a different physiological state such as bioluminescence, virulent gene expression, and bio-film formation.
In Vibrio fischeri, when the population density (and hence the concentration of autoinducer) crosses a certain threshold, the expression of a set of genes that is required for bioluminescence is turned on. The production of the autoinducer is under the control of a gene, the expression of which involves positive feedback. The following figure shows the various components of this system (Ref. 1).
