Paris/September 18
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*S26: (lRBS-DapAsubtilis) MPT5.? (préciser David) | *S26: (lRBS-DapAsubtilis) MPT5.? (préciser David) | ||
*pKD13: (plasmide FRT-KanR-FRT de Wanner), un stock glycerol est également réalisé | *pKD13: (plasmide FRT-KanR-FRT de Wanner), un stock glycerol est également réalisé | ||
| + | |||
| + | |||
| + | == Digestion reactions == | ||
| + | |||
| + | {| | ||
| + | |- style="background: #ccccff;" | ||
| + | ! colspan="5" style="background: #ccccff;" | Digestion Products | ||
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |width=5%| Number | ||
| + | |width=24%| Product Name | ||
| + | |width=24%| Matrix Name | ||
| + | |width=5%| Enzyme 1 | ||
| + | |width=5%| Enzyme 2 | ||
| + | |width=5%| Size | ||
| + | |width=34%| Description | ||
| + | |width=1%| | ||
| + | |width=1%| | ||
| + | |width=1%| | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |D77 | ||
| + | |araC/pBad>>lox71-gfptripart BI | ||
| + | |D60 (.1 & .2) digestion product | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #FF99FF;" |D78 | ||
| + | |araC/pBad>>lox71-gfptripart FI | ||
| + | |D71 (.1 & .2) digestion product | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |D53 | ||
| + | |RBS-dapAsubtilis BBinsert | ||
| + | |pTOPO of RBS-dapAsubtilis (MPT5.2) | ||
| + | |EcoRI | ||
| + | |Pst1 | ||
| + | | | ||
| + | |RBS-dapAsubtilis extracted for cloning as a biobrick | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |D54 | ||
| + | |RBS-dapAsubtilis BI | ||
| + | |pTOPO of RBS-dapAsubtilis (MPT5.1) | ||
| + | |XbaI | ||
| + | |Pst1 | ||
| + | | | ||
| + | |RBS-dapAsubtilis extracted for use as a BI | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |} | ||
Revision as of 10:14, 18 September 2007
Minipreps
Minipreps sur cultures ON de:
- S24: (lox66-DapAsubtilis) MPT3.? (préciser David)
- S26: (lRBS-DapAsubtilis) MPT5.? (préciser David)
- pKD13: (plasmide FRT-KanR-FRT de Wanner), un stock glycerol est également réalisé
Digestion reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D77 | araC/pBad>>lox71-gfptripart BI | D60 (.1 & .2) digestion product | in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||||||
| D78 | araC/pBad>>lox71-gfptripart FI | D71 (.1 & .2) digestion product | in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||||||
| D53 | RBS-dapAsubtilis BBinsert | pTOPO of RBS-dapAsubtilis (MPT5.2) | EcoRI | Pst1 | RBS-dapAsubtilis extracted for cloning as a biobrick | ||||
| D54 | RBS-dapAsubtilis BI | pTOPO of RBS-dapAsubtilis (MPT5.1) | XbaI | Pst1 | RBS-dapAsubtilis extracted for use as a BI | ||||
