Paris/September 18
From 2007.igem.org
(Difference between revisions)
Eismoustique (Talk | contribs) (→Digestion reactions) |
Eismoustique (Talk | contribs) (→Digestion reactions) |
||
| Line 28: | Line 28: | ||
|araC/pBad>>lox71-gfptripart BI | |araC/pBad>>lox71-gfptripart BI | ||
|D60 (.1 & .2) digestion product | |D60 (.1 & .2) digestion product | ||
| - | | | + | |FspI |
| - | | | + | |AhdI |
| | | | ||
|in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | |in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||
| Line 39: | Line 39: | ||
|araC/pBad>>lox71-gfptripart FI | |araC/pBad>>lox71-gfptripart FI | ||
|D71 (.1 & .2) digestion product | |D71 (.1 & .2) digestion product | ||
| - | | | + | |FspI |
| - | | | + | |AhdI |
| | | | ||
|in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | |in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||
Revision as of 10:28, 18 September 2007
Minipreps
Minipreps sur cultures ON de:
- S24: (lox66-DapAsubtilis) MPT3.? (préciser David)
- S26: (lRBS-DapAsubtilis) MPT5.? (préciser David)
- pKD13: (plasmide FRT-KanR-FRT de Wanner), un stock glycerol est également réalisé
Digestion reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D77 | araC/pBad>>lox71-gfptripart BI | D60 (.1 & .2) digestion product | FspI | AhdI | in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||||
| D78 | araC/pBad>>lox71-gfptripart FI | D71 (.1 & .2) digestion product | FspI | AhdI | in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion | ||||
| D53 | RBS-dapAsubtilis BBinsert | pTOPO of RBS-dapAsubtilis (MPT5.2) | EcoRI | Pst1 | RBS-dapAsubtilis extracted for cloning as a biobrick | ||||
| D54 | RBS-dapAsubtilis BI | pTOPO of RBS-dapAsubtilis (MPT5.2) | XbaI | Pst1 | RBS-dapAsubtilis extracted for use as a BI | ||||
