Paris/September 18

From 2007.igem.org

(Difference between revisions)
(→Digestion reactions)
Line 67: Line 67:
|
|
|
|
 +
|
 +
|}
 +
 +
 +
 +
== Ligation reactions ==
 +
{|
 +
|- style="background: #ccccff;"
 +
! colspan="5" style="background: #ccccff;" | Ligations
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| Number
 +
|width=20%| Insert
 +
|width=5%| Insert Volume (µL)
 +
|width=25%| Vector
 +
|width=5%| Vector Volume (µL)
 +
|width=35%| Comments
 +
|width=5%| Number of colonies
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |C1
 +
|
 +
|
 +
|MP3.1 digested with EcoRI (D0)
 +
|2
 +
|control of ligation efficiency
|
|
|}
|}

Revision as of 16:10, 18 September 2007

Minipreps

Minipreps sur cultures ON de:

  • S24: (lox66-DapAsubtilis) MPT3.? (préciser David)
  • S26: (lRBS-DapAsubtilis) MPT5.? (préciser David)
  • pKD13: (plasmide FRT-KanR-FRT de Wanner), un stock glycerol est également réalisé


Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D77 araC/pBad>>lox71-gfptripart BI D60 (.1 & .2) digestion product FspI AhdI in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
D78 araC/pBad>>lox71-gfptripart FI D71 (.1 & .2) digestion product FspI AhdI in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
D53 RBS-dapAsubtilis BBinsert pTOPO of RBS-dapAsubtilis (MPT5.2) EcoRI Pst1 RBS-dapAsubtilis extracted for cloning as a biobrick
D54 RBS-dapAsubtilis BI pTOPO of RBS-dapAsubtilis (MPT5.2) XbaI Pst1 RBS-dapAsubtilis extracted for use as a BI


Ligation reactions

Ligations
Number Insert Insert Volume (µL) Vector Vector Volume (µL) Comments Number of colonies
C1 MP3.1 digested with EcoRI (D0) 2 control of ligation efficiency
Retrieved from "http://2007.igem.org/wiki/index.php/Paris/September_18"