Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner. | We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner. | ||
+ | {| border="1" | ||
+ | !Enzyme!!Source!!Recognition Sequence!!Cut | ||
+ | |- | ||
+ | |[[EcoRI|''Eco''RI]]||''[[Escherichia coli]]'' | ||
+ | | | ||
+ | 5'GAATTC | ||
+ | 3'CTTAAG | ||
+ | | | ||
+ | 5'---G AATTC---3' | ||
+ | 3'---CTTAA G---5' | ||
+ | |- | ||
+ | |[[XbaI|''Xba''I]]<ref name=h/>||''[[Xanthomonas badrii]]'' | ||
+ | | | ||
+ | 5'TCTAGA | ||
+ | 3'AGATCT | ||
+ | | | ||
+ | 5'---T CTAGA---3' | ||
+ | 3'---AGATC T---5' | ||
+ | |- | ||
+ | |colspan=4|* = blunt ends | ||
+ | |- | ||
+ | |[[PstI|''Pst''I]]<ref name=h/>||''[[Providencia stuartii]]'' | ||
+ | | | ||
+ | 5'CTGCAG | ||
+ | 3'GACGTC | ||
+ | | | ||
+ | 5'---CTGCA G---3' | ||
+ | 3'---G ACGTC---5' | ||
+ | |- | ||
+ | |} |
Revision as of 11:34, 20 September 2007
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
Enzyme | Source | Recognition Sequence | Cut |
---|---|---|---|
EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
XbaI<ref name=h/> | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
* = blunt ends | |||
PstI<ref name=h/> | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |