Biobrick Primers Design
From 2007.igem.org
(Difference between revisions)
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| - | ''FOX3 XbaI Fw'' | + | '''''FOX3 XbaI Fw''''' |
5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ | 5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ | ||
Revision as of 13:32, 20 September 2007
FOX3 XbaI Fw
5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
Lungh=21
Tm 60°C
FOX3 SpeI Rev
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
Lungh=19
Tm 50°C
Luciferase Xba I Fv
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
Lungh=22
Tm 60°C
Luciferase Spe I Rv
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
Lungh=20
Tm 60°C
2XORE – CYC1 XbaI Fw
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
Lungh=22
Tm 58,4°C
1X ORE – CYC1 XbaI Fw
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
Lungh=18
Tm 56°C
2&1ORE-CYC1 Spe I Rev
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
Lungh=18
Tm 56°C
