Biobrick Primers Design
From 2007.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
Tm 60°C | Tm 60°C | ||
| - | + | ''' | |
| - | ''FOX3 SpeI Rev'' | + | '''''FOX3 SpeI Rev'' |
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’ | 5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’ | ||
| Line 15: | Line 15: | ||
Tm 50°C | Tm 50°C | ||
| - | ''Luciferase Xba I Fv'' | + | '''''Luciferase Xba I Fv''''' |
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’ | 5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’ | ||
| Line 23: | Line 23: | ||
Tm 60°C | Tm 60°C | ||
| - | ''Luciferase Spe I Rv'' | + | '''''Luciferase Spe I Rv'' ''' |
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ | 5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ | ||
| Line 31: | Line 31: | ||
Tm 60°C | Tm 60°C | ||
| - | 2XORE – CYC1 XbaI Fw | + | '''''2XORE – CYC1 XbaI Fw''''' |
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’ | 5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’ | ||
| Line 39: | Line 39: | ||
Tm 58,4°C | Tm 58,4°C | ||
| - | ''1X ORE – CYC1 XbaI Fw'' | + | '''''1X ORE – CYC1 XbaI Fw''''' |
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’ | 5’- GC TCTAGA GAGATGGCTCGACCATCT-3’ | ||
| Line 47: | Line 47: | ||
Tm 56°C | Tm 56°C | ||
| - | ''2&1ORE-CYC1 Spe I Rev'' | + | '''''2&1ORE-CYC1 Spe I Rev''''' |
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’ | 5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’ | ||
Revision as of 13:33, 20 September 2007
FOX3 XbaI Fw
5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
Lungh=21
Tm 60°C FOX3 SpeI Rev
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
Lungh=19
Tm 50°C
Luciferase Xba I Fv
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
Lungh=22
Tm 60°C
Luciferase Spe I Rv
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
Lungh=20
Tm 60°C
2XORE – CYC1 XbaI Fw
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
Lungh=22
Tm 58,4°C
1X ORE – CYC1 XbaI Fw
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
Lungh=18
Tm 56°C
2&1ORE-CYC1 Spe I Rev
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
Lungh=18
Tm 56°C
