Boston University

From 2007.igem.org

Revision as of 22:37, 29 May 2007

About Us

Welcome to the wiki for Boston University's iGEM 2007 team!

Our team members are consists of the following members:

David Shi, Rahul Ahuja, Christian Ling, Danny Bellin.

We are all soon-to-be juniors majoring in Biomedical Engineering.

We are advised by [http://www.bu.edu/dbin/bme/faculty/?prof=tgardner Dr. Timothy Gardner], Assistant Professor of Biomedical Engineering, as well as Frank Juhn and Stephen Schneider, students at the [http://www.gardnerlab.bu.edu Gardner Laboratory], where we work.

Our Project Plan

Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors

Our plan so far:

1. Mutate global transcription factors
   1. Design primers for amplification
   2. Perform error-prone PCR
2. Transform mutated genes into E. coli
   1. Choose plasmids
   2. Restriction enzyme digestion
   3. Ligation
   4. Transformation into E. coli
3. Conjugate E. coli with Shewanella
4. Screen/select for increased current production due to mutations

Week's Goals

Wednesday 5/30

1. Get all protocols
2. Identify materials/prepare order
3. Design Primers
4. Learn about budget/POs

Thursday 5/31

1. Do primer order
2. Start conjugation practice
3. Confirm restriction enzymes, ligases
4. Order confirmed/needed materials
5. Team Revew Meeting

Friday 6/1

1. Evaluate/continue conjugation, practice electroporation for E. coli
2. Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads