Boston University
From 2007.igem.org
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## Transformation into E. coli | ## Transformation into E. coli | ||
# Conjugate E. coli with Shewanella | # Conjugate E. coli with Shewanella | ||
| - | # Screen/select for increased current production due to mutations | + | # Screen/select for increased current production due to mutations. Potential methods: |
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== Week's Goals == | == Week's Goals == | ||
Revision as of 22:39, 29 May 2007
About Us
Welcome to the wiki for Boston University's iGEM 2007 team!
Our team consists of David Shi, Rahul Ahuja, Christian Ling, and Danny Bellin, all soon-to-be juniors majoring in Biomedical Engineering at Boston University.
We are advised by [http://www.bu.edu/dbin/bme/faculty/?prof=tgardner Dr. Timothy Gardner], Assistant Professor of Biomedical Engineering, as well as Frank Juhn and Stephen Schneider, students in the [http://www.gardnerlab.bu.edu Gardner Laboratory], where we work.
Our Project Plan
Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors.
Our plan so far:
- Mutate global transcription factors
- Transform mutated genes into E. coli
- Choose plasmids
- Restriction enzyme digestion
- Ligation
- Transformation into E. coli
- Conjugate E. coli with Shewanella
- Screen/select for increased current production due to mutations. Potential methods:
Week's Goals
Wednesday 5/30
- Get all protocols
- Identify materials/prepare order
- Design Primers
- Learn about budget/POs
Thursday 5/31
- Do primer order
- Start conjugation practice
- Confirm restriction enzymes, ligases
- Order confirmed/needed materials
- Team Revew Meeting
Friday 6/1
- Evaluate/continue conjugation, practice electroporation for E. coli
- Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads
