NYMU Taipei/Lab Notes/2007 9 27
From 2007.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
** 1Kb ladder | ** 1Kb ladder | ||
** block #1 (insert band appears) | ** block #1 (insert band appears) | ||
| - | ** block #2 (insert band appears, and one | + | ** block #2 (insert band appears, and one extra band around 400 bp) |
| - | ** block #3 () | + | ** block #3 (insert band appears) |
| - | ** block #4 | + | ** block #4 (insert band appears) |
| - | ** block #5 | + | ** block #5 (insert band does not appear, only band around 400 bp) |
| - | ** block #6 | + | ** block #6 (insert band does not appear, only band around 400 bp) |
* expect results: one band at 1,623 bp is our insert | * expect results: one band at 1,623 bp is our insert | ||
** insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163) | ** insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163) | ||
** insert = 790 + 730 + 103 = 1,623 | ** insert = 790 + 730 + 103 = 1,623 | ||
Revision as of 09:11, 27 September 2007
- After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate
- PCR check using VF2, VR primers
- PCR total 50 uL, 10 uL for GEL separation
- sample at each lane
- 1Kb ladder
- block #1 (insert band appears)
- block #2 (insert band appears, and one extra band around 400 bp)
- block #3 (insert band appears)
- block #4 (insert band appears)
- block #5 (insert band does not appear, only band around 400 bp)
- block #6 (insert band does not appear, only band around 400 bp)
- expect results: one band at 1,623 bp is our insert
- insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
- insert = 790 + 730 + 103 = 1,623
