From 2007.igem.org

Revision as of 02:26, 31 May 2007 (view source) Dbellin (Talk | contribs) (→Week's (Ambitious) Goals) ← Older edit		Revision as of 02:27, 31 May 2007 (view source) Dbellin (Talk | contribs) (→Materials We Need) Newer edit →
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	Ligases:  Need to Buy?		Ligases:  Need to Buy?
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		+	== Short-Term To-Do List ==
		+
		+	Lab Orientation: COMPLETED!
		+	*[[Lab Orientation Checklist]]
		+
		+	Lab Safety Training: Not Completed (Dave, Rahul, and Christian are scheduled for a training session on 5/30/07.  Danny will be taking it next week)
		+
		+	Design of Primers:  Not Completed (Dave, please send me info about the finished primers when ready)
		+
		+	Ordering of Primers:  Not Completed
		+
		+	Gathering of Protocols:  Not Completed (Chris, please send me the protocols when they are gathered)
		+
		+	Ordering of Error-Prone PCR Materials:  Not completed
		+
		+	Thank-You Letters sent to Pfizer:  COMPLETED!
		+
		+	Thank-You Letters sent to BU ppl:  Not completed

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Revision as of 02:27, 31 May 2007

Week's (Ambitious) Goals

Wednesday 5/30

1. Get all protocols
2. Identify materials/prepare order
3. Design Primers
4. Learn about budget/POs

Thursday 5/31

1. Do primer order
2. Start conjugation practice
3. Confirm restriction enzymes, ligases
4. Order confirmed/needed materials
5. Team Revew Meeting
6. Draft Thank-You Letters for our Sponsors

Friday 6/1

1. Evaluate/continue conjugation, practice electroporation for E. coli
2. Revise proposal to include possibility of screening with alginate beads and fluorocytometer
3. Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads

Materials We Need

Primers: Need to Buy

Error-Prone PCR: Need to Buy

Plasmids: Need to Buy?

Restriction Enzymes: Need to Buy?

Ligases: Need to Buy?

Short-Term To-Do List

Lab Orientation: COMPLETED!

• Lab Orientation Checklist

Lab Safety Training: Not Completed (Dave, Rahul, and Christian are scheduled for a training session on 5/30/07. Danny will be taking it next week)

Design of Primers: Not Completed (Dave, please send me info about the finished primers when ready)

Ordering of Primers: Not Completed

Gathering of Protocols: Not Completed (Chris, please send me the protocols when they are gathered)

Ordering of Error-Prone PCR Materials: Not completed

Thank-You Letters sent to Pfizer: COMPLETED!

Thank-You Letters sent to BU ppl: Not completed