Melbourne/Secondary Reagent Agar Plates

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< Melbourne(Difference between revisions)
 
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[[Melbourne/Protocols for Secondary Reagents|<return to list of secondary methods>]]  [[Melbourne|  <team home page>]]
[[Melbourne/Protocols for Secondary Reagents|<return to list of secondary methods>]]  [[Melbourne|  <team home page>]]
===Preparation of Agar Plates===
===Preparation of Agar Plates===
 +
===Uses===
===Uses===
 +
*Plating out and separating bacterial colonies
 +
===Ingedients:===
===Ingedients:===
-
*
+
*[[Melbourne/Secondary Reagent LB|LB]]
 +
*[[Melbourne/primary AMP|Ampicillin]]
 +
*[[Melbourne/primary agar|Agar]]
 +
*Petri Dishes
 +
 
 +
 
===Method:===
===Method:===
-
#
+
#Add 1.4g of [[Melbourne/primary agar|Agar]] per 100mL of [[Melbourne/Secondary Reagent LB|LB]]
 +
#Autoclave (just enough to melt)
 +
#Cool down to about 40-50 degrees C on bench/cold room/water.
 +
#Add 1/1000 volume of [[Melbourne/primary AMP|Ampicillin]].
 +
#Mix and pour into petri dishes. 100ml makes 3-4 plates.
 +
#Leave plates to dry on bench/laminar flow partly covered.
 +
#Keep in cold room till used
 +
 
 +
===Equipment:===
 +
*Autoclave or Microwave

Latest revision as of 08:42, 29 September 2007

<return to list of secondary methods> <team home page>

Contents

Preparation of Agar Plates

Uses

  • Plating out and separating bacterial colonies

Ingedients:


Method:

  1. Add 1.4g of Agar per 100mL of LB
  2. Autoclave (just enough to melt)
  3. Cool down to about 40-50 degrees C on bench/cold room/water.
  4. Add 1/1000 volume of Ampicillin.
  5. Mix and pour into petri dishes. 100ml makes 3-4 plates.
  6. Leave plates to dry on bench/laminar flow partly covered.
  7. Keep in cold room till used

Equipment:

  • Autoclave or Microwave
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