Toronto/Lab Notebook

From 2007.igem.org

(Difference between revisions)

Revision as of 00:44, 4 October 2007

Oct. 3, 2007

Yusuf

SUMMARY:

Transformed: J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
Length Check:

| Magnitude | | | | | amount | op | total() | print() | | 1000 | + | 1000 | [1000 USD] | | 1000 | + | 2000 | [2000 USD] | | 10000000000 | + | 10000002000 | [10000002000 USD]| | 3000 | - | 9999999000 | [9999999000 USD]|

TO DO:

Make 5 Kan plates
Make competent cells
Make o/n of J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
Ligate N1 + S01003

Oct. 2, 2007

Natalie

SUMMARY:

Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
NOTE: N1 = J34100 C + S01640 B + B0015

TO DO:

Miniprep all above parts and length check
Transform:
- J06801 (2005) - it's in the source box (this is a larger composite part containing J06501)
- B0034 - also in source box, if not, get it from Registry plates
- Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
- J31005 - chloramphenicol resistance, just in case we need it
Continue cataloging and organization (Charles: I'll be dropping by to help out with that)

Oct. 1, 2007

Time: 3:45 PM - 8:30 PM

Yusuf, Irina

Summary:

made 5 KAN plates
made 2 AMP+KAN plates
made 300 mL LB

Tranformation of following parts:

I13033 -> KAN
E0433 -> KAN
S03140 -> AMP
J04500 -> KAN
J31003 -> AMP
(J23100C + S01640B)+ B0015 -> KAN

Used 4uL of DNA for all transformations

tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
couldn't find it in the igem dna box as well !!!

TO DO:

MP o/n for the above parts

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