From 2007.igem.org

Revision as of 01:18, 4 October 2007 (view source) Chuck (Talk | contribs) ← Older edit		Revision as of 01:21, 4 October 2007 (view source) Chuck (Talk | contribs) (→Oct. 3, 2007) Newer edit →
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	* Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB		* Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
	* Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)		* Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
-	* Length Check:	+	* Length Check (o = match, x = does not match):
	<table border=1>		<table border=1>
	<tr><td>'''Part Name'''<td>'''Part'''<td>'''Plasmid'''</tr>		<tr><td>'''Part Name'''<td>'''Part'''<td>'''Plasmid'''</tr>
-	<tr><td>E0433 A<td>o<td>o</tr>	+	<tr><td>E0433 A<td><td></tr>
		+	<tr><td>E0433  B<td><td></tr>
		+	<tr><td>I13033 A<td><td></tr>
		+	<tr><td>I13033 B<td><td></tr>
		+	<tr><td>J04500 A<td><td></tr>
		+	<tr><td>J04500 B<td><td></tr>
		+	<tr><td>J31003 A<td><td></tr>
		+	<tr><td>J31003 B<td><td></tr>
		+	<tr><td>N1    A<td><td></tr>
		+	<tr><td>N1    B<td><td></tr>
		+	<tr><td>S03140 A<td><td></tr>
		+	<tr><td>S03140 B<td><td></tr>
	</table>		</table>

---

Revision as of 01:21, 4 October 2007

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Oct. 3, 2007

Yusuf

SUMMARY:

• Transformed: J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
• Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
• Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
• Length Check (o = match, x = does not match):

Part Name	Part	Plasmid
E0433 A
E0433 B
I13033 A
I13033 B
J04500 A
J04500 B
J31003 A
J31003 B
N1 A
N1 B
S03140 A
S03140 B

TO DO:

• Make 5 Kan plates
• Make bottle of LB Broth
• Make competent cells
• Make o/n of J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
• Ligate N1 + S01003

Oct. 2, 2007

Natalie

SUMMARY:

• Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
• NOTE: N1 = J34100 C + S01640 B + B0015

TO DO:

• Miniprep all above parts and length check
• Transform:
  • J06801 (2005) - it's in the source box (this is a larger composite part containing J06501)
  • B0034 - also in source box, if not, get it from Registry plates
  • Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
  • J31005 - chloramphenicol resistance, just in case we need it
• Continue cataloging and organization (Charles: I'll be dropping by to help out with that)

Oct. 1, 2007

Time: 3:45 PM - 8:30 PM

Yusuf, Irina

Summary:

• made 5 KAN plates
• made 2 AMP+KAN plates
• made 300 mL LB

Tranformation of following parts:

• I13033 -> KAN
• E0433 -> KAN
• S03140 -> AMP
• J04500 -> KAN
• J31003 -> AMP
• (J23100C + S01640B)+ B0015 -> KAN

Used 4uL of DNA for all transformations

• tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
• couldn't find it in the igem dna box as well !!!

TO DO:

• MP o/n for the above parts