Valencia/October
From 2007.igem.org
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we have done the gel of the PCR reactions, but it looks like the DNA has not amplified our samples, but the reaction has worked fine for the controls. | we have done the gel of the PCR reactions, but it looks like the DNA has not amplified our samples, but the reaction has worked fine for the controls. | ||
| - | we repeat the PCRs and make some other controls with different DNA quantities. | + | we repeat the PCRs and make some other controls with different DNA quantities. the gels shows some bands, but their weights do not correspond to anything we wanted... could be some other ''lab dwarf''?? |
| - | we have miniprepped the inoculations that have grown overnight. this way, we will have more DNA to | + | we have miniprepped the inoculations that have grown overnight. this way, we will have more DNA to sequence |
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| + | ===3/10/07=== | ||
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| + | we have digested the plasmid were we did the PCRs with EcoRI and PstI | ||
---- | ---- | ||
===<div align="right">[[Valencia/November|November →]]</div>=== | ===<div align="right">[[Valencia/November|November →]]</div>=== | ||
Revision as of 11:30, 4 October 2007
Work Progress
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← September
1/10/07
we leave some overnight PCRs reactions, using VF2 and VR, in order to properly analyze our clones...
at the same time, we are inoculating all those clones in order to make some minipreps.
2/10/07
we have done the gel of the PCR reactions, but it looks like the DNA has not amplified our samples, but the reaction has worked fine for the controls.
we repeat the PCRs and make some other controls with different DNA quantities. the gels shows some bands, but their weights do not correspond to anything we wanted... could be some other lab dwarf??
we have miniprepped the inoculations that have grown overnight. this way, we will have more DNA to sequence
3/10/07
we have digested the plasmid were we did the PCRs with EcoRI and PstI
