From 2007.igem.org

Revision as of 14:45, 4 October 2007 (view source) Blent (Talk | contribs) (→Re-check the concentration of inserts and vectors) ← Older edit		Revision as of 14:45, 4 October 2007 (view source) Blent (Talk | contribs) (→Re-check the concentration of inserts and vectors) Newer edit →
Line 34:		Line 34:
	</tr>		</tr>
	</table>		</table>
		+	== ligation setup==

---

Revision as of 14:45, 4 October 2007

digestion check of CinR+HSL+D-term,pCinRHSL and pOmpC (vectors)

lane A: 1kb ladder lane B: CinR+HSL+D-term x 2 lane C: 1kb ladder lane D: pCinRHSL x 2 lane E: pOmpC x 2

• after gel separation, their O.D. was checked. However, it is too low

Re-check the concentration of inserts and vectors

lane A: 1kb ladder lane B: pCinRHSL lane C: pOmpC lane D: CinR+HSL+D-term lane E: 100bp ladder lane F: TATA_INSA lane G: TATB_INSB lane H: OmpRBS

Owing to concentrations of vectors are too low and A260/A280 ratios are not correct (maybe too much ions) thus, we checked the vectors with inserts by PCR to decide how to ligate them

ligation setup