NYMU Taipei/Lab Notes/2007 10 5
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
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== PCR length check == | == PCR length check == | ||
* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR | * PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR | ||
| - | * expected lengths of three ligations | + | * expected combined insertion lengths of three ligations |
** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613 | ** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613 | ||
*** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65) | *** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65) | ||
Latest revision as of 05:17, 9 October 2007
PCR length check
- PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
- expected combined insertion lengths of three ligations
- (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
- 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)
- (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
- 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)
-
(III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145
- (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
