From 2007.igem.org

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	'''[rbs][ATG]''' <br>		'''[rbs][ATG]''' <br>
		+	'''4 Different rbs variants (1106A, 1106B, 1128A, 1090)''' <br>
-	Digest pBca1101-Bca1106 (BglII/AlwnI, 2062 + 127bp, Small) <br>	+	----
-	Digest pBca9145-Bca1112 (BamHI/AlwnI, 1528 + 553bp, Large) <br>	+
		+
		+	Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br>
		+	Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br>
	Product is pBca9145-I716201. <br>		Product is pBca9145-I716201. <br>
		+
		+	Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br>
		+	Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br>
		+	Product is pBca9145-I716202. <br>
		+
		+	Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large) <br>
		+	Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br>
		+	Product is pBca9145-I716203. <br>
		+
		+	Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large) <br>
		+	Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br>
		+	Product is pBca9145-I716204. <br>

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Revision as of 18:27, 4 June 2007

[rbs][ATG]
4 Different rbs variants (1106A, 1106B, 1128A, 1090)

---

Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716201.

Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716202.

Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716203.

Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716204.