Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

(Difference between revisions)
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*32.5ul ddH<sub>2</sub>O
*32.5ul ddH<sub>2</sub>O
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+
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50ul Total
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*50ul Total

Revision as of 14:45, 10 October 2007

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

  • 5ul 10x buffer
  • 5ul Enhancer
  • 0.6ul dNTPs (25mM stock)
  • 2.5ul MgSO4 (Supplied in PCR kit)
  • 1.5ul Primer I (10uM)
  • 1.5ul Primer II (10uM)
  • 1ul Template (pJS010)
  • 0.4ul Pfx Platinum (Invitrogen)
  • 32.5ul ddH2O

--

  • 50ul Total
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