Melbourne/Lab BL Notebook/PCR1
From 2007.igem.org
(Difference between revisions)
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32.5ul ddH<sub>2</sub>O | 32.5ul ddH<sub>2</sub>O | ||
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50ul Total | 50ul Total | ||
Revision as of 14:47, 10 October 2007
=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
PCR mix
5ul 10x buffer\\
5ul Enhancer
0.6ul dNTPs (25mM stock)
2.5ul MgSO4 (Supplied in PCR kit)
1.5ul Primer I (10uM)
1.5ul Primer II (10uM)
1ul Template (pJS010)
0.4ul Pfx Platinum (Invitrogen)
32.5ul ddH2O
---
50ul Total
